Emergence of bidirectional cell laning from collective contact guidance

Abstract

Directed collective cell migration is central in morphogenesis, wound healing and cancer progression1,2. Although it is well-accepted that the molecular anisotropy of the micro-environment guides this migration3,4, its impact on the pattern of the cell flows remains largely unexplored. Studying confluent human bronchial epithelial cells (HBECs) in vitro, we show that subcellular microgrooves elicit a polar mode of collective migration in millimeter-long bidirectional lanes that are much wider than a cell size, even though cell flows are highly disordered on featureless surfaces 5. This directed flocking-like transition6,7 can be accounted for by a hydrodynamic theory of active polar fluids and corresponding numerical simulations. This model further predicts that anisotropic friction resulting from the grooves lowers the threshold of the transition, which we confirm experimentally. Therefore, microscopic anisotropy of the environment not only directs the collective motion of the cells in the easy direction, but also shapes the cell migration pattern. Flow patterns induced by collective contact guidance are thus markedly different from those induced by supracellular confinement8, demonstrating that all length-scales of the micro-environment must be considered in a comprehensive description of collective migration. Furthermore, artificial microtopographies designed from theoretical considerations can provide a rational strategy to direct cells to specific geometries and functions, which has broad implications, for instance, for tissue engineering strategies in organoid morphogenesis.