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arXiv · 2407.20469

Efficient, gigapixel-scale, aberration-free whole slide scanner using angular ptychographic imaging with closed-form solution

Shi Zhao·Haowen Zhou·Siyu Lin·Ruizhi Cao·Changhuei Yang
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Abstract

Whole slide imaging provides a wide field-of-view (FOV) across cross-sections of biopsy or surgery samples, significantly facilitating pathological analysis and clinical diagnosis. Such high-quality images that enable detailed visualization of cellular and tissue structures are essential for effective patient care and treatment planning. To obtain such high-quality images for pathology applications, there is a need for scanners with high spatial bandwidth products, free from aberrations, and without the requirement for z-scanning. Here we report a whole slide imaging system based on angular ptychographic imaging with a closed-form solution (WSI-APIC), which offers efficient, tens-of-gigapixels, large-FOV, aberration-free imaging. WSI-APIC utilizes oblique incoherent illumination for initial high-level segmentation, thereby bypassing unnecessary scanning of the background regions and enhancing image acquisition efficiency. A GPU-accelerated APIC algorithm analytically reconstructs phase images with effective digital aberration corrections and improved optical resolutions. Moreover, an auto-stitching technique based on scale-invariant feature transform ensures the seamless concatenation of whole slide phase images. In our experiment, WSI-APIC achieved an optical resolution of 772 nm using a 10x/0.25 NA objective lens and captures 80-gigapixel aberration-free phase images for a standard 76.2 mm x 25.4 mm microscopic slide.

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Shi Zhao, Haowen Zhou, Siyu Lin, Ruizhi Cao, Changhuei Yang. 2024-07-29. Efficient, gigapixel-scale, aberration-free whole slide scanner using angular ptychographic imaging with closed-form solution. https://arxiv.org/abs/2407.20469

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