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arXiv · 2408.00578

Propagation of Enzyme-driven Active Fluctuations in Crowded Milieu

Rik Chakraborty·Arnab Maiti·Diptangshu Paul·Rajnandan Borthakur·K. R. Jayaprakash·Uddipta Ghosh·Krishna Kanti Dey
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Abstract

We investigated the energy transfer from active enzymes to their surroundings in crowded environments by measuring the diffusion of passive microscopic tracers in active solutions of ficoll and glycerol. Despite observing lower rates of substrate turnover and relatively smaller enhancement of passive tracer diffusion in artificial crowded media compared to those in aqueous solutions, we found a significantly higher relative diffusion enhancement in crowded environments in the presence of enzymatic activity. Our experimental observations, coupled with supporting analytical estimations, underscored the critical role of the intervening media in facilitating mechanical energy distribution around active enzymes.

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Rik Chakraborty, Arnab Maiti, Diptangshu Paul, Rajnandan Borthakur, K. R. Jayaprakash, Uddipta Ghosh, Krishna Kanti Dey. 2024-08-01. Propagation of Enzyme-driven Active Fluctuations in Crowded Milieu. https://arxiv.org/abs/2408.00578

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