Searcharxiv⌕ Search
Home/Archive/arXiv

arXiv · 2411.04232

Invitro Pharmacological Evaluations Of Ethanolic Extract Of Jatropha Maheshwari

Sankar V·Anand Babu K·Deepak M·Poojitha Mallapu·Raghu S·Anandharaj G
View original source ↗Read source document ↗Export metadata ↓

Abstract

Objective: To assess the antioxidant, wound healing, anti-ulcer, and anti-inflammatory properties of Jatropha maheshwari. Methods: Jatropha maheshwari was collected from Kanyakumari district and authenticated. Ethanol was used for continuous hot percolation extraction of the plant. Antioxidant activity was evaluated using DPPH and ABTS assays. The wound healing potential was assessed through a wound scratch assay using 3T3-L1 murine fibroblast cell lines. Anti-ulcer activity was measured using the acid-neutralizing capacity (ANC) and H+/K+-ATPase inhibitory activity methods. Anti-inflammatory effects were determined through dose-dependent studies of the ethanol extract. Results: Antioxidant Activity: The crude extract exhibited strong antioxidant capacity with percentage inhibition values of 88% (DPPH) and 75.7% (ABTS). Wound Healing Activity: The wound closure rate in treated 3T3-L1 cell lines reached 97.88%, indicating potent wound healing properties. Anti-Ulcer Activity: The extract demonstrated an 80.1% inhibition compared to the control when tested alongside omeprazole. ANC per gram of antacid was recorded at 20 and 26 for oral doses of 10 and 500 {\mu}g/ml, respectively. Anti-Inflammatory Activity: Dose-dependent inhibition percentages of 30.1% (10 {\mu}g/ml) and 96.5% (500 {\mu}g/ml) were observed (p<0.001) relative to inflammation control. Additional percentages of 26.3% (10 {\mu}g/ml) and 42.6% (500 {\mu}g/ml) further confirmed the anti-inflammatory activity. Conclusion: Jatropha maheshwari demonstrates significant antioxidant, wound healing, anti-ulcer, and anti-inflammatory properties. Further research is warranted to elucidate the mechanisms underlying these pharmacological effects.

Explore related subjects

q-bio.QMq-bio.CB

Keep this discovery

BibTeXRIS

Sankar V, Anand Babu K, Deepak M, Poojitha Mallapu, Raghu S, Anandharaj G. 2024-11-05. Invitro Pharmacological Evaluations Of Ethanolic Extract Of Jatropha Maheshwari. https://arxiv.org/abs/2411.04232

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related papers

arXiv2026

Biology-in-the-loop: Amortized Adaptive Hit Discovery in CRISPR Screens

Many biological discovery problems require experiments to be selected sequentially under constrained budgets. CRISPR screening is a prominent example, as exhaustive perturbation testing is often infeasible and candidate perturbations must instead be prioritized over multiple experimental rounds. Despite the importance of this problem, existing benchmarks for adaptive hit discovery remain limited in scale and diversity. Here, we introduce AssayBench-Loop, a large-scale benchmark for adaptive hit discovery comprising 1,389 CRISPR screens across five phenotype categories. Beyond enabling systematic evaluation, its scale makes it possible to learn acquisition strategies across historical experiments. Building on this resource, we introduce AssayLoop, a sequential experimental design framework combining AssayFormer, a transformer-based amortized acquisition policy trained across historical screens to adapt from experimental feedback, with LLM-derived biological priors through an adaptive handoff. In this view, completed experiments become training data for learning how accumulated evidence should guide what to test next, while LLMs provide prior biological knowledge to seed the search. We further introduce AssayLLM, showing that the same principle can be extended directly to an LLM through task-specific post-training. On temporally held-out screens, AssayLoop achieves a 5.67-fold enrichment over random selection and recovers 27.7% of hits after assaying approximately 5% of the candidate library, outperforming existing adaptive-design methods and standalone LLMs, and AssayFormer alone. Performance improves with increasing historical training data and transfers to phenotype categories excluded from training. These results demonstrate the value of learning acquisition policies across historical experiments and combining them with broad biological priors for efficient adaptive hit discovery.

q-bio.QM↗
arXiv2026

Multi-Task Bacterial Colony Detection and Classification Using YOLOv8 with Edge Optimization for Resource-Constrained Deployment

Manual counting and classification of bacterial colonies are critical yet labor-intensive tasks in microbiology, prone to human error particularly on densely populated plates. This work proposes a multi-task deep learning framework trained on the Annotated Germs for Automated Recognition (AGAR) dataset (18,000 images; 9,202 training / 3,067 testing) to automate Colony Forming Unit (CFU) enumeration and species classification. A custom multi-task CNN employing global regression served as the baseline, but demonstrated limited performance in clustered colony environments due to the absence of spatial localization. To address this, a YOLOv8 object detection architecture was adopted with high-resolution 1024x1024 inputs, enabling instance-level colony detection and label assignment. The model achieved a classification accuracy of 98.13% and a counting accuracy of 98.27% (within a 10-colony margin), demonstrating strong predictive capability. To bridge the gap between model performance and practical deployability, the trained model was optimized through unstructured and structured pruning, ONNX conversion, and reduced-precision inference (FP32, FP16, INT8). On a Raspberry Pi 4B, ONNX FP32 and FP16 variants offered the best balance between inference speed (~6.4s) and accuracy (MAE ~2.20). Unstructured pruning preserved predictive accuracy (MAE ~2.01) without runtime gains, while structured pruning resulted in significant accuracy degradation (MAE ~6.3), revealing the sensitivity of instance-level colony detection to architectural compression. These findings provide practical guidance for selecting optimization strategies in resource-constrained laboratory deployments.

q-bio.QM↗
arXiv2026

ADMET-EvO: a self-evolving scientific agent for sustained research across heterogeneous tasks

Scientific agents can move beyond automated model building by using accumulated evidence to revise both their questions and experimental strategies. The challenge is sustaining this adaptation across heterogeneous tasks without overfitting decisions to internal validation. Absorption, distribution, metabolism, excretion and toxicity (ADMET) prediction provides a demanding setting across diverse assays, datasets and chemical domains. We therefore developed ADMET-EvO, an evidence-gated agent that formalizes endpoints, generates falsifiable hypotheses and tests interventions across data, feature and model axes. It carries supported, rejected and inconclusive outcomes forward to guide each new cycle. Across the 22-task Therapeutics Data Commons (TDC) ADMET benchmark, ADMET-EvO achieved the highest task-normalized score of 96.77. Evidence-guided selection reduced cumulative fitting time by 72.2% within a predefined non-inferiority margin. It also formalized 43 toxicity-related tasks and constructed endpoint-specific predictors. Together, these results show how ADMET-EvO can accumulate evidence, revise its strategy and expand its research scope over time.

q-bio.QM↗