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arXiv · 2502.03667

Sample Motion for Structured Illumination Fluorescence Microscopy

Ruiming Cao·Guanghan Meng·Laura Waller
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Abstract

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single speckle illumination pattern and relying on inherent sample motion to encode the super-resolved information in multiple raw images. From a set of raw fluorescence images captured as the sample moves, we jointly estimate both the sample motion and the super-resolved image. We demonstrate the feasibility of the proposed method both in simulation and in experiment.

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Ruiming Cao, Guanghan Meng, Laura Waller. 2025-02-05. Sample Motion for Structured Illumination Fluorescence Microscopy. https://doi.org/10.1364/ol.560873

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