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arXiv · 2502.16672

Super-resolution Live-cell Fluorescence Lifetime Imaging

Raphaël Marchand·Henning Ortkrass·Daniel Aziz·Franz Pfanner·Eman Abbas·Silvio O. Rizzoli·Wolfgang Hübner·Adam Bowman·Thomas Huser·Thomas Juffmann
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Abstract

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM makes it readily compatible with live-cell imaging. Here, we combine SR-SIM with electro-optic fluorescence lifetime imaging (EOFLIM), adding the capability of monitoring physicochemical parameters with 156 nm spatial resolution at high frame rate for live-cell imaging. We demonstrate that our new SIMFLIM technique enables super-resolved multiplexed imaging of spectrally overlapping fluorophores, environmental sensing, and live-cell imaging.

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Raphaël Marchand, Henning Ortkrass, Daniel Aziz, Franz Pfanner, Eman Abbas, Silvio O. Rizzoli, Wolfgang Hübner, Adam Bowman, Thomas Huser, Thomas Juffmann. 2025-02-23. Super-resolution Live-cell Fluorescence Lifetime Imaging. https://arxiv.org/abs/2502.16672

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