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arXiv · 2509.23847

13CFLUX -- Third-generation high-performance engine for isotopically (non)stationary 13C metabolic flux analysis

Anton Stratmann·Martin Beyß·Johann F. Jadebeck·Wolfgang Wiechert·Katharina Nöh
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Abstract

13C-based metabolic flux analysis (13C-MFA) is a cornerstone of quantitative systems biology, yet its increasing data complexity and methodological diversity place high demands on simulation software. We introduce 13CFLUX(v3), a third-generation simulation platform that combines a high-performance C++ engine with a convenient Python interface. The software delivers substantial performance gains across isotopically stationary and nonstationary analysis workflows, while remaining flexible to accommodate diverse labeling strategies and analytical platforms. Its open-source availability facilitates seamless integration into computational ecosystems and community-driven extension. By supporting multi-experiment integration, multi-tracer studies, and advanced statistical inference such as Bayesian analysis, 13CFLUX provides a robust and extensible framework for modern fluxomics research.

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Anton Stratmann, Martin Beyß, Johann F. Jadebeck, Wolfgang Wiechert, Katharina Nöh. 2025-09-28. 13CFLUX -- Third-generation high-performance engine for isotopically (non)stationary 13C metabolic flux analysis. https://arxiv.org/abs/2509.23847

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