Generating Synthetic Human Blastocyst Images for In-Vitro Fertilization Blastocyst Grading

Abstract

The success of in vitro fertilization (IVF) at many clinics relies on the accurate morphological assessment of day 5 blastocysts, a process that is often subjective and inconsistent. While artificial intelligence can help standardize this evaluation, models require large, diverse, and balanced datasets, which are often unavailable due to data scarcity, natural class imbalance, and privacy constraints. Existing generative embryo models can mitigate these issues but face several limitations, such as poor image quality, small training datasets, non-robust evaluation, and lack of clinically relevant image generation for effective data augmentation. Here, we present the Diffusion Based Imaging Model for Artificial Blastocysts (DIA) framework, a set of latent diffusion models trained to generate high-fidelity, novel day 5 blastocyst images. Our models provide granular control by conditioning on Gardner-based morphological categories and z-axis focal depth. We rigorously evaluated the models using FID, a memorization metric, an embryologist Turing test, and three downstream classification tasks. Our results show that DIA models generate realistic images that embryologists could not reliably distinguish from real images. Most importantly, we demonstrated clear clinical value. Augmenting an imbalanced dataset with synthetic images significantly improved classification accuracy (p < 0.05). Also, adding synthetic images to an already large, balanced dataset yielded statistically significant performance gains, and synthetic data could replace up to 40% of real data in some cases without a statistically significant loss in accuracy. DIA provides a robust solution for mitigating data scarcity and class imbalance in embryo datasets. By generating novel, high-fidelity, and controllable synthetic images, our models can improve the performance, fairness, and standardization of AI embryo assessment tools.