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arXiv · 2601.01267

Integrated vortex-assisted electroporation platform with enhanced throughput for genetic delivery to primary cells

Hyun Woo Sung·Soojung Claire Hur
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Abstract

Primary human cells offer the most faithful representation of native human physiology, yet their practical utility is constrained by the difficulty of introducing exogenous genetic material. Electroporation provides a promising non-viral gene delivery approach; however, conventional bulk systems lack the uniformity and integration required for heterogeneous primary cell samples. Here, we present a vortex-assisted electroporation platform integrating size-selective cell trapping with enhanced throughput, parameter optimization across buffer and electrical conditions, and robust delivery of plasmid DNA and in vitro-transcribed mRNA in primary human cells. This integrated platform provides a unified workflow that addresses sample heterogeneity, throughput demands, and delivery efficiency, enabling broader implementation of non-viral gene delivery into primary cells for research and translational applications.

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BibTeXRIS

Hyun Woo Sung, Soojung Claire Hur. 2026-01-03. Integrated vortex-assisted electroporation platform with enhanced throughput for genetic delivery to primary cells. https://doi.org/10.3390/mi17030359

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