Constituintes quimicos e atividades biologicas das folhas e cascas do caule de ficus maxima mill. (moraceae)

Abstract

Ficus maxima (Moraceae, subgenus Pharmacosycea), known in Brazil as "caxinguba", occurs in northern Brazil and in Mato Grosso. Its leaves and fruits are important food sources for birds and mammals, and the species is traditionally used by Indigenous peoples of Central and South America to treat intestinal parasites, gingivitis, internal inflammation, and snakebites. Despite its medicinal relevance, studies on its chemistry and biological properties are still limited. This work aimed to characterize the chemical constituents of F. maxima leaves and stem bark using UPLC-MS-MS and to evaluate the antinociceptive activity and CYP1A inhibition of ethanolic extracts and fractions. Plant material collected in Abaetetuba, Para (October 2013), was extracted and fractionated by silica gel column chromatography. UPLC-MS-MS analyses in positive and negative ion modes enabled putative metabolite identification supported by MS/MS libraries and molecular networking on GNPS. A total of 45 metabolites were identified, including flavonoids, triterpenes, coumarins, polyunsaturated fatty acids, amino acids, and alkaloids. Major constituents in the leaf extract included triterpene 51, flavonoids 75 and 70, and alkaloid 29. The stem bark ethanolic extract showed marked antinociceptive activity in the inflammatory phase of the formalin test (muscarinic pathway) and 62.6 +/- 9.2% activity in the hot-plate test (opioid pathway). The dichloromethane fraction of the leaves exhibited potent CYP1A1 inhibitory activity in vitro. These findings support the traditional use of F. maxima as an anti-inflammatory resource and associate its major constituents with the observed activities, reinforcing its potential as a therapeutic source that deserves further investigation.