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arXiv · 2602.16327

Guide-Guard: Off-Target Predicting in CRISPR Applications

Joseph Bingham·Netanel Arussy·Saman Zonouz
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Abstract

With the introduction of cyber-physical genome sequencing and editing technologies, such as CRISPR, researchers can more easily access tools to investigate and create remedies for a variety of topics in genetics and health science (e.g. agriculture and medicine). As the field advances and grows, new concerns present themselves in the ability to predict the off-target behavior. In this work, we explore the underlying biological and chemical model from a data driven perspective. Additionally, we present a machine learning based solution named \textit{Guide-Guard} to predict the behavior of the system given a gRNA in the CRISPR gene-editing process with 84\% accuracy. This solution is able to be trained on multiple different genes at the same time while retaining accuracy.

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Joseph Bingham, Netanel Arussy, Saman Zonouz. 2026-02-18. Guide-Guard: Off-Target Predicting in CRISPR Applications. https://doi.org/10.1007/978-3-031-21753-1_41

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