Micropatterning photopolymerizable hydrogels for diffusion studies using pillar arrays or photomasks

Abstract

In situ polymerization and micropatterning of hydrogels on-chip opens the potential for many applications such as tracking and controlling the diffusion of molecules, stimulants, inhibitors, as well as nutrients and drugs, from their source to a target. To enable such applications, we developed hydrogel-on chip platforms for molecular diffusion studies by refining PEGDA-PEG hydrogel in terms of micropatterning and diffusion properties. In the first platform that we introduce here, the design has multiple adjacent microfluidic channels separated with pillar arrays shaping the flow of our custom-prepared photopolymerizable hydrogels and thus enabling the localization of photopolymerization. In the second platform, a photomask formation has been achieved by coupling the micro-milling of 250-\textmu m thickness of PMMA substrate with Platinum (Pt)-coating onto the PMMA mask. In this way the design was obtained to have opaque and transparent regions for light-based polymerization of the PEGDA-PEG hydrogels. The developed method of in situ polymerization of the hydrogel on-chip through photomask has enabled direct transfer of the design of interest to the pre-hydrogel in channel. Next, the developed platforms will be further used to test various compositions of photopolymerizable hydrogels and track and control the diffusion of molecules across hydrogel interfaces. The micropatterning methods and platforms developed here could be tailored to device design and development needs in various application fields from molecular transport to biosensing to electronic devices.