Noninvasive H3 K27M screening in pediatric diffuse midline glioma using radiomics on heterogeneous T2-weighted MRI

Arthur Zagitov; Alexander Beznosikov; Vladimir Bozhenko; Ninel Kamyshnikova; Tatiana Kulinich; Sofia Polozova; Yaroslav Kholodov

arXiv · 2607.01497

Noninvasive H3 K27M screening in pediatric diffuse midline glioma using radiomics on heterogeneous T2-weighted MRI

Arthur Zagitov·Alexander Beznosikov·Vladimir Bozhenko·Ninel Kamyshnikova·Tatiana Kulinich·Sofia Polozova·Yaroslav Kholodov

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Abstract

Histone H3K27M mutation status defines a clinically aggressive subgroup of pediatric diffuse midline glioma and informs prognosis and trial eligibility, but confirmation usually requires tissue sampling from eloquent midline structures. We evaluated whether radiomics from routinely available T2-weighted MRI can provide an adjunctive screening signal in a heterogeneous referral-style cohort, where scans are often acquired externally and T2-weighted imaging is the only consistently available sequence. Ninety-eight pediatric patients with tissue-confirmed status were analyzed (73 mutation-positive, 25 wild-type). Expert tumor segmentations defined the regions of interest for PyRadiomics feature extraction after isotropic resampling, dual skull stripping, and multi-scale filtering. We systematically ablated preprocessing, correlation pruning with repeated recursive feature elimination, tumor volume, and TabDDPM synthetic minority augmentation across 100 stratified train/test splits with real-only test sets. Pure radiomics achieved accuracy 0.664 and F1-score 0.784. The best pipeline used preprocessing, feature selection, and volume with CatBoost, achieving accuracy 0.730$\pm$0.068 and F1-score 0.826$\pm$0.044. TabDDPM improved TabPFN to F1-score 0.81$\pm$0.05 at 200 augmented rows. These results support T2-weighted radiomics as a moderate screening and triage aid, not a replacement for tissue-based diagnosis.

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Arthur Zagitov, Alexander Beznosikov, Vladimir Bozhenko, Ninel Kamyshnikova, Tatiana Kulinich, Sofia Polozova, Yaroslav Kholodov. 2026-07-01. Noninvasive H3 K27M screening in pediatric diffuse midline glioma using radiomics on heterogeneous T2-weighted MRI. https://arxiv.org/abs/2607.01497

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