On the interpretation of the kinetics of ligand-receptor binding

David Colquhoun; James P Higham

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arXiv · 2609.01228

On the interpretation of the kinetics of ligand-receptor binding

David Colquhoun·James P Higham

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Abstract

When the rates of ligand binding are measured by methods such as surface plasmon resonance, it is common practice to use the observed rate constants for the onset and offset of binding to estimate an equilibrium constant for ligand binding. If this agrees with the equilibrium constant found as the EC50 for binding at equilibrium, this is taken as validation of the measured rates. This is correct only when binding produces no conformation change in the receptor, and ligand binding follows a single exponential time course. Here, we investigate a simple 3 state model in which binding is followed by a conformation change in the receptor. Three special cases of this model in which the time course of onset and offset of ligand binding are close to being single exponentials are analysed. These cases are (1) when binding is much faster than the conformation change, (2) when the conformation change is much faster than binding, and (3) when the rates of ligand dissociation and receptor activation are both fast. It is concluded that the measured rates will often yield an estimate of the equilibrium constant for ligand binding that is close to the effective, or macroscopic, equilibrium constant, the EC50 found by measuring binding at equilibrium, which depends on both of the underlying microscopic equilibrium constants describing ligand binding and the conformation change. The exception to this conclusion is the case when binding is much faster than the subsequent conformation change, though the estimate of the equilibrium constant for ligand binding still depends on both of the underlying microscopic equilibrium constants.

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David Colquhoun, James P Higham. 2026-09-01. On the interpretation of the kinetics of ligand-receptor binding. https://arxiv.org/abs/2609.01228

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