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Measurements of Protein-Protein Interactions by Size Exclusion Chromatography

J. Bloustine·V. Berejnov·S. Fraden
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Abstract

A method is presented for determining second virial coefficients B_2 of protein solutions from retention time measurements in size exclusion chromatography (SEC). We determine B_2 by analyzing the concentration dependance of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B_2 from positive to negative values in lysozyme and bovine serum albumin solutions. Our SEC results agree quantitatively with data obtained by light scattering.

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J. Bloustine, V. Berejnov, S. Fraden. 2003-06-12. Measurements of Protein-Protein Interactions by Size Exclusion Chromatography. https://doi.org/10.1016/s0006-3495(03)74684-0

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