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Impact of Short- and Long-range Forces on Protein Conformation and Adsorption Kinetics

Anthony Quinn·Hubert Mantz·Karin Jacobs·Markus Bellion·Ludger Santen
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Abstract

We have studied the adsorption kinetics of the protein amylase at solid/liquid interfaces. Offering substrates with tailored properties, we are able to separate the impact of short- and long-range interactions. By means of a colloidal Monte Carlo approach including conformational changes of the adsorbed proteins induced by density fluctuations, we develop a scenario that is consistent with the experimentally observed three-step kinetics on specific substrates. Our observations show that not only the surface chemistry determines the properties of an adsorbed protein layer but also the van der Waals contributions of a composite substrate may lead to non-negligible effects.

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Anthony Quinn, Hubert Mantz, Karin Jacobs, Markus Bellion, Ludger Santen. 2007-05-16. Impact of Short- and Long-range Forces on Protein Conformation and Adsorption Kinetics. https://arxiv.org/abs/cond-mat/0607384

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