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Kinetic model of DNA replication in eukaryotic organisms

John Herrick·Suckjoon Jun·John Bechhoefer·Aaron Bensimon
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Abstract

We formulate a kinetic model of DNA replication that quantitatively describes recent results on DNA replication in the in vitro system of Xenopus laevis prior to the mid-blastula transition. The model describes well a large amount of different data within a simple theoretical framework. This allows one, for the first time, to determine the parameters governing the DNA replication program in a eukaryote on a genome-wide basis. In particular, we have determined the frequency of origin activation in time and space during the cell cycle. Although we focus on a specific stage of development, this model can easily be adapted to describe replication in many other organisms, including budding yeast.

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John Herrick, Suckjoon Jun, John Bechhoefer, Aaron Bensimon. 2001-01-25. Kinetic model of DNA replication in eukaryotic organisms. https://doi.org/10.1016/s0022-2836(02)00522-3

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