SearcharxivSearch

arXiv subjects

Alexis Gautreau

Publications and source records attributed to Alexis Gautreau.

2 recordsLinked to original sources

Que r\'ev\`ele l'activit\'e de validation de d\'emonstration circulaire sur la compr\'ehension de d\'emonstration

This communication contributes to research on proof validation as a lens for uncovering didactical phenomena related to proof and proving. We revisit the puzzling case of lower secondary students in France who validate circular proofs. That is proofs in which the conclusion is used within a step of the proof itself. While these 12--13-year-old students accurately identify the final statement of such proofs as a reformulation of the conclusion of the claim, they encounter difficulties in interpreting how the condition of the claim is taken up within the proof. Our analysis challenges Miyazaki and al.'s interpretation of this phenomenon, which attributes students' acceptation of the validity of circular reasoning to a misunderstanding of modus ponens when there are two in a row. Instead, we propose an alternative explanation grounded in the distinction between the operative and theoretical statuses of mathematical propositions. This distinction provides both a theoretical rationale for the invalidity of circular proofs and a didactical tool for examining students' argumentative activity when they attempt to reject such proofs.

math.HO

A highly specific gold nanoprobe for live-cell single-molecule imaging

Single molecule tracking in live cells is the ultimate tool to study subcellular protein dynamics, but it is often limited by the probe size and photostability. Due to these issues, long-term tracking of proteins in confined and crowded environments, such as intracellular spaces, remains challenging. We have developed a novel optical probe consisting of 5-nm gold nanoparticles functionalized with a small fragment of camelid antibodies that recognize widely used GFPs with a very high affinity, which we call GFP-nanobodies. These small gold nanoparticles can be detected and tracked using photothermal imaging for arbitrarily long periods of time. Surface and intracellular GFP-proteins were effectively labeled even in very crowded environments such as adhesion sites and cytoskeletal structures both in vitro and in live cell cultures. These nanobody-coated gold nanoparticles are probes with unparalleled capabilities; small size, perfect photostability, high specificity, and versatility afforded by combination with the vast existing library of GFP-tagged proteins.

physics.bio-ph