arXiv subjects

Austin Van Namen

Publications and source records attributed to Austin Van Namen.

3 recordsLinked to original sources

arXiv2025

Deep Learning-Driven Quantitative Spectroscopic Photoacoustic Imaging for Segmentation and Oxygen Saturation Estimation

Spectroscopic photoacoustic (sPA) imaging can potentially estimate blood oxygenation saturation (sO2) in vivo noninvasively. However, quantitatively accurate results require accurate optical fluence estimates. Robust modeling in heterogeneous tissue, where light with different wavelengths can experience significantly different absorption and scattering, is difficult. In this work, we developed a deep neural network (Hybrid-Net) for sPA imaging to simultaneously estimate sO2 in blood vessels and segment those vessels from surrounding background tissue. sO2 error was minimized only in blood vessels segmented in Hybrid-Net, resulting in more accurate predictions. Hybrid-Net was first trained on simulated sPA data (at 700 nm and 850 nm) representing initial pressure distributions from three-dimensional Monte Carlo simulations of light transport in breast tissue. Then, for experimental verification, the network was retrained on experimental sPA data (at 700 nm and 850 nm) acquired from simple tissue mimicking phantoms with an embedded blood pool. Quantitative measures were used to evaluate Hybrid-Net performance with an averaged segmentation accuracy of >= 0.978 in simulations with varying noise levels (0dB-35dB) and 0.998 in the experiment, and an averaged sO2 mean squared error of <= 0.048 in simulations with varying noise levels (0dB-35dB) and 0.003 in the experiment. Overall, these results show that Hybrid-Net can provide accurate blood oxygenation without estimating the optical fluence, and this study could lead to improvements in in-vivo sO2 estimation.

eess.IV↗

arXiv2023

Multiplex ultrasound imaging of perfluorocarbon nanodroplets enabled by decomposition of post-vaporization dynamics

Among the various molecular imaging modalities, ultrasound imaging benefits from its real-time, nonionizing, and cost-effective nature. Despite its benefits, there is a dearth of methods to visualize two or more populations of contrast agents simultaneously, a technique known as multiplex imaging. In this paper, we present a new approach to multiplex ultrasound imaging using perfluorocarbon (PFC) nanodroplets. The nanodroplets, which undergo a liquid-to-gas phase transition in response to an acoustic trigger, act as activatable contrast agents. By using two populations of PFC nanodroplets, each with a different core boiling point, their unique temporal responses to an acoustic trigger were leveraged to differentiate their unique contributions to the overall ultrasound signal. This work characterized the dynamic responses of two PFC nanodroplets with boiling points of 28 and 56 °C. These characteristic responses were then used to demonstrate that the relative concentrations of the two populations of PFC nanodroplets could be accurately measured in the same imaging volume within an average error of 1.1%. Overall, the findings indicate the potential of this approach for multiplex ultrasound imaging, allowing for the visualization of multiple molecular targets simultaneously.

eess.IV↗

arXiv2021

Repeated Acoustic Vaporization of Perfluorohexane Nanodroplets for Contrast-Enhanced Ultrasound Imaging

Superheated perfluorocarbon nanodroplets are emerging ultrasound imaging contrast agents boasting biocompatible components, unique phase-change dynamics, and therapeutic loading capabilities. Upon exposure to a sufficiently high intensity pulse of acoustic energy, the nanodroplet perfluorocarbon core undergoes a liquid-to-gas phase change and becomes an echogenic microbubble, providing ultrasound contrast. The controllable activation leads to high-contrast images, while the small size of the nanodroplets promotes longer circulation times and better in-vivo stability. One drawback, however, is that the nanodroplets can only be vaporized a single time, limiting their versatility. Recently, we and others have address this issue by using a perfluorohexane core, which has a boiling point above body temperature. Thus after vaporization, the microbubbles recondense back into their stable nanodroplets form. Previous work with perfluorohexane nanodroplets relied on optical activation via pulse laser absorption of an encapsulated dye. This strategy limits the imaging depth and temporal resolution of the method. In this study we overcome these limitations by demonstrating acoustic droplet vaporization with 1.1-MHz high intensity focused ultrasound. A short-duration, high-amplitude pulse of focused ultrasound provides a sufficiently strong peak negative pressure to initiate vaporization. When using a custom imaging sequence with a high-frequency transducer, the repeated acoustic activation of perfluorohexane nanodroplets can be visualized in polyacrylamide tissue-mimicking phantoms. We demonstrate detection of hundreds of vaporization events from individual nanodroplets with activation thresholds well below the tissue cavitation limit. Overall, this approach has the potential to result in reliable contrast-enhanced ultrasound imaging at clinically relevant depths.

physics.med-ph↗