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Baoli Yao

Publications and source records attributed to Baoli Yao.

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Scalable Generalized Meta-Spanners Enabling Parallel Multitasking Optical Manipulation

Optical manipulation techniques offer exceptional contactless control but are fundamentally limited in their ability to perform parallel multitasking. To achieve high-density, versatile manipulation with subwavelength photonic devices, it is essential to sculpt light fields in multiple dimensions. Here, we overcome this challenge by introducing generalized optical meta-spanners (GOMSs) based on metasurfaces. Relying on complex-amplitude modulation, this platform generates lens-free, customizable optical fields that suppress diffractive losses. As a result, several advanced functionalities are simultaneously achieved, including longitudinally varying manipulation and in-plane spanner arrays, which outperforms the same operations realized by conventional donut-shaped orbital flows. Furthermore, the particle dynamics is reconfigurable simply by switching the input and output polarizations, facilitating robust multi-channel control. We experimentally validate the proposed approach by demonstrating single-particle dynamics and the parallel manipulation of particle ensembles, revealing exceptional stability for multitasking operations. These results demonstrate an ultracompact platform scalable to a much larger number of optical spanners, advancing metadevices from wavefront sculptors to particle manipulators. We envision that the GOMS will catalyze innovations in cross-disciplinary fields such as targeted drug delivery and cell-level biomechanics.

physics.optics

Optical intensity-gradient torque due to chiral multipole interplay

Owing to the ubiquity and easy-to-shape property of optical intensity, the intensity gradient force of light has been most spectacularly exploited in optical manipulation of small particles. Manifesting the intensity gradient as an optical torque to spin particles is of great fascination on both fundamental and practical sides but remains elusive. Here, we uncover the existence of the optical intensity-gradient torque in the interaction of light with chiral particles. Such a new type of torque derives from the interplay between chirality induced multipoles, which switches its direction for particles with opposite chirality. We show that this torque can be directly detected by a simple standing wave field, created with the interference of two counterpropagating plane-like waves. Our work offers a unique route to achieve rotational control of matter by tailoring the field intensity of Maxwell waves. It also establishes a framework that maps a remarkable connection among the optical forces and torques, across chiral to nonchiral.

physics.optics

Edge effect removal in Fourier ptychographic microscopy via periodic plus smooth image decomposition

Fourier ptychographic microscopy (FPM) is a promising computational imaging technique with high resolution, wide field-of-view (FOV) and quantitative phase recovery. So far, a series of system errors that may corrupt the image quality of FPM has been reported. However, an imperceptible artifact caused by edge effect caught our attention and may also degrade the precision of phase imaging in FPM with a cross-shape artifact in the Fourier space. We found that the precision of reconstructed phase at the same subregion depends on the different sizes of block processing as a result of different edge conditions, which limits the quantitative phase measurements via FPM. And this artifact is caused by the aperiodic image extension of fast Fourier transform (FFT). Herein, to remove the edge effect and improve the accuracy, two classes of opposite algorithms termed discrete cosine transform (DCT) and perfect Fourier transform (PFT) were reported respectively and discussed systematically. Although both approaches can remove the artifacts in FPM and may be extended to other Fourier analysis techniques, PFT has a comparable efficiency to conventional FFT. The PFT algorithm improves the standard deviation of phase accuracy as a factor of 4 from 0.08 radians to 0.02 radians. Finally, we summarized and discussed all the reported system errors of FPM within a generalized model.

eess.IV

Observation of high-order imaginary Poynting momentum optomechanics in structured light

The imaginary Poynting momentum (IPM) of light has been captivated an unusual origin of optomechanical effects on dipolar magnetoelectric particles, but yet observed in experiments. Here, we report, for the very first time, a whole family of high-order IPM forces for not only magnetoelectric but also generic Mie particles, assisted with their excited higher multipoles within. Such optomechanical phenomena derive from a nonlinear contribution of the IPM to the optical force, and can be remarkable even when the incident IPM is small. We observe the high-order optomechanics in a structured light beam with vortex-like IPM streamlines, which allows the low-order dipolar contribution to be suppressed. Our results provide the first unambiguous evidence of the ponderomotive nature of the IPM, expand the classification of optical forces and open new possibilities for optical forces and micromanipulations.

physics.optics

High-throughput fast full-color digital pathology based on Fourier ptychographic microscopy via color transfer

Full-color imaging is significant in digital pathology. Compared with a grayscale image or a pseudo-color image that only contains the contrast information, it can identify and detect the target object better with color texture information. Fourier ptychographic microscopy (FPM) is a high-throughput computational imaging technique that breaks the tradeoff between high resolution (HR) and large field-of-view (FOV), which eliminates the artifacts of scanning and stitching in digital pathology and improves its imaging efficiency. However, the conventional full-color digital pathology based on FPM is still time-consuming due to the repeated experiments with tri-wavelengths. A color transfer FPM approach, termed CFPM was reported. The color texture information of a low resolution (LR) full-color pathologic image is directly transferred to the HR grayscale FPM image captured by only a single wavelength. The color space of FPM based on the standard CIE-XYZ color model and display based on the standard RGB (sRGB) color space were established. Different FPM colorization schemes were analyzed and compared with thirty different biological samples. The average root-mean-square error (RMSE) of the conventional method and CFPM compared with the ground truth is 5.3% and 5.7%, respectively. Therefore, the acquisition time is significantly reduced by 2/3 with the sacrifice of precision of only 0.4%. And CFPM method is also compatible with advanced fast FPM approaches to reduce computation time further.

eess.IV

Coherent synthetic aperture imaging for visible remote sensing via reflective Fourier ptychography

Synthetic aperture radar (SAR) can measure the phase with antenna and microwave, which cannot be directly extended to visible light imaging due to phase lost. In this letter, we reported an active remote sensing with visible light via reflective Fourier ptychography (FP), termed coherent synthetic aperture imaging (CSAI), achieving high resolution, wide field-of-view (FOV) and phase recovery. A proof-of-concept experiment was reported with laser scanning and a collimator for the infinite object. Both smooth and rough objects are tested, and the spatial resolution increased from 15.6 um to 3.48 um with a factor of 4.5. The speckle noise can be suppressed by FP unexpectedly. Meanwhile, the CSAI method may replace the adaptive optics to tackle the aberration induced from atmospheric turbulence and optical system by one-step deconvolution.

physics.optics

In situ correction of liquid meniscus in cell culture imaging system based on parallel Fourier ptychographic microscopy (96 Eyes)

We collaborated with Amgen and spent five years in designing and fabricating next generation multi-well plate imagers based on Fourier ptychographic microscopy (FPM). A 6-well imager (Emsight) and a low-cost parallel microscopic system (96 Eyes) based on parallel FPM were reported in our previous work. However, the effect of liquid meniscus on the image quality is much stronger than anticipated, introducing obvious wavevector misalignment and additional image aberration. To this end, an adaptive wavevector correction (AWC-FPM) algorithm and a pupil recovery improvement strategy are presented to solve these challenges in situ. In addition, dual-channel fluorescence excitation is added to obtain structural information for microbiologists. Experiments are demonstrated to verify their performances. The accuracy of angular resolution with our algorithm is within 0.003 rad. Our algorithms would make the FPM algorithm more robust and practical and can be extended to other FPM-based applications to overcome similar challenges.

physics.bio-ph

Retrieval of non-sparse object through scattering media beyond the memory effect

Optical imaging through scattering media is a commonly confronted with the problem of reconstruction of complex objects and optical memory effect. To solve the problem, here, we propose a novel configuration based on the combination of ptychography and shower-curtain effect, which enables the retrieval of non-sparse samples through scattering media beyond the memory effect. Furthermore, by virtue of the shower-curtain effect, the proposed imaging system is insensitive to dynamic scattering media. Results from the retrieval of hair follicle section demonstrate the effectiveness and feasibility of the proposed method. The field of view is improved to 2.64mm. This present technique will be a potential approach for imaging through deep biological tissue.

eess.IV

Subwavelength resolution Fourier ptychography with hemispherical digital condensers

Fourier ptychography (FP) is a promising computational imaging technique that overcomes the physical space-bandwidth product (SBP) limit of a conventional microscope by applying angular diversity illuminations. However, to date, the effective imaging numerical aperture (NA) achievable with a commercial LED board is still limited to the range of 0.3 to 0.7 with a 4X0.1 NA objective due to the constraint of planar geometry with weak illumination brightness and attenuated signal-to-noise ratio (SNR). Thus the highest achievable half-pitch resolution is usually constrained between 500 to 1000 nm, which cannot fulfill some needs of high-resolution biomedical imaging applications. Although it is possible to improve the resolution by using a higher magnification objective with larger NA instead of enlarging the illumination NA, the SBP is suppressed to some extent, making the FP technique less appealing, since the reduction of field-of-view (FOV) is much larger than the improvement of resolution in this FP platform. Herein, in this paper, we initially present a subwavelength resolution Fourier ptychography (SRFP) platform with a hemispherical digital condenser to provide high-angle programmable plane-wave illuminations of 0.95NA, attaining a 4X0.1 NA objective with the final effective imaging performance of 1.05NA at a half-pitch resolution of 244 nm with a wavelength of 465 nm across a wide FOV of 14.60 mm2, corresponding to an SBP of 245 megapixels. Our work provides an essential step of FP towards high-NA imaging applications without scarfing the FOV, making it more practical and appealing.

physics.optics

Vignetting effect in Fourier ptychographic microscopy

Fourier ptychographic microscopy (FPM) is a computational imaging technique that overcomes the physical space-bandwidth product (SBP) limit of a conventional microscope by applying angular diversity illuminations. In the usual model of FPM, the microscopic system is approximated by being taken as space-invariant with transfer function determined by a complex pupil function of the objective. However, in real experimental conditions, several unexpected "semi-bright and semi-dark" images with strong vignetting effect can be easily observed when the sample is illuminated by the LED within the "transition zone" between bright field and dark field. These imperfect images, apparently, are not coincident with the space-invariant model and could deteriorate the reconstruction quality severely. In this Letter, we examine the impact of this space-invariant approximation on FPM image formation based on ray-based and rigorous wave optics-based analysis. Our analysis shows that for a practical FPM microscope with a low power objective and a large field of view, the space invariance is destroyed by diffraction at other stops associated with different lens elements to a large extent. A modified version of the space-variant model is derived and discussed. Two simple countermeasures are also presented and experimentally verified to bypass or partially alleviate the vignetting-induced reconstruction artifacts.

physics.optics

SNR-based adaptive acquisition method for fast Fourier ptychographic microscopy

Fourier ptychographic microscopy (FPM) is a computational imaging technique with both high resolution and large field-of-view. However, the effective numerical aperture (NA) achievable with a typical LED panel is ambiguous and usually relies on the repeated tests of different illumination NAs. The imaging quality of each raw image usually depends on the visual assessments, which is subjective and inaccurate especially for those dark field images. Moreover, the acquisition process is really time-consuming.In this paper, we propose a SNR-based adaptive acquisition method for quantitative evaluation and adaptive collection of each raw image according to the signal-to-noise ration (SNR) value, to improve the FPM's acquisition efficiency and automatically obtain the maximum achievable NA, reducing the time of collection, storage and subsequent calculation. The widely used EPRY-FPM algorithm is applied without adding any algorithm complexity and computational burden. The performance has been demonstrated in both USAF targets and biological samples with different imaging sensors respectively, which have either Poisson or Gaussian noises model. Further combined with the sparse LEDs strategy, the number of collection images can be shorten to around 25 frames while the former needs 361 images, the reduction ratio can reach over 90%. This method will make FPM more practical and automatic, and can also be used in different configurations of FPM.

eess.IV

Data preprocessing methods for robust Fourier ptychographic microscopy

Fourier ptychographic microscopy (FPM) is a recently proposed computational imaging technique with both high resolution and wide field-of-view. In current FP experimental setup, the dark-field images with high-angle illuminations are easily submerged by stray light and background noise due to the low signal-to-noise ratio, thus significantly degrading the reconstruction quality and also imposing a major restriction on the synthetic numerical aperture (NA) of the FP approach. To this end, an overall and systematic data preprocessing scheme for noise removal from FP's raw dataset is provided, which involves sampling analysis as well as underexposed/overexposed treatments, then followed by the elimination of unknown stray light and suppression of inevitable background noise, especially Gaussian noise and CCD dark current in our experiments. The reported non-parametric scheme facilitates great enhancements of the FP's performance, which has been demonstrated experimentally that the benefits of noise removal by these methods far outweigh its defects of concomitant signal loss. In addition, it could be flexibly cooperated with the existing state-of-the-art algorithms, producing a stronger robustness of the FP approach in various applications.

cs.CV

System calibration method for Fourier ptychographic microscopy

Fourier ptychographic microscopy (FPM) is a recently proposed quantitative phase imaging technique with high resolution and wide field-of-view (FOV). In current FPM imaging platforms, systematic error sources come from the aberrations, LED intensity fluctuation, parameter imperfections and noise, which will severely corrupt the reconstruction results with artifacts. Although these problems have been researched and some special methods have been proposed respectively, there is no method to solve all of them. However, the systematic error is a mixture of various sources in the real situation. It is difficult to distinguish a kind of error source from another due to the similar artifacts. To this end, we report a system calibration procedure, termed SC-FPM, based on the simulated annealing (SA) algorithm, LED intensity correction and adaptive step-size strategy, which involves the evaluation of an error matric at each iteration step, followed by the re-estimation of accurate parameters. The great performance has been achieved both in simulation and experiments. The reported system calibration scheme improves the robustness of FPM and relaxes the experiment conditions, which makes the FPM more pragmatic.

physics.ins-det