arXiv subjects

Bernard Lorber

Publications and source records attributed to Bernard Lorber.

2 recordsLinked to original sources

arXiv2018

Analytical light scattering methods in molecular and structural biology: Experimental aspects and results

Non-invasive light scattering methods provide data on biological macromolecules (i.e. proteins, nucleic acids, as well as assemblies and larger entities composed of them) that are complementary with those of size exclusion chromatography, gel electrophoresis, analytical ultracentrifugation and mass spectrometry methods. Static light scattering measurements are useful to determine the mass of macromolecules and to monitor aggregation phenomena. Dynamic light scattering measurements are suitable for the quality control and to assess sample homogeneity, to determine particle size, examine the effect of physical and chemical treatments, probe the binding of ligands, and study interactions between macromolecules.

q-bio.BM↗

arXiv2017

Better results with homogeneous biological macromolecules

Pure and homogeneous biological macromolecules (i.e. proteins, nucleic acids, protein-protein or protein-nucleic acid complexes, and functional assemblies such as ribosomes and viruses) are the key for consistent and reliable biochemical and biophysical measurements, as well as for reproducible crystallizations, best crystal diffraction properties, and exploitable electron microscopy images. Highlights: Pure and homogeneous macromolecules are the key for the best experimental results; They warrant the consistency and the reliability of biochemical and biophysical data; They give more reproducible crystallography and electron microscopy results as well.

q-bio.BM↗