Biaobin Jiang | Searcharxiv

arXiv2025

Fast and Accurate Antibody Sequence Design via Structure Retrieval

Recent advancements in protein design have leveraged diffusion models to generate structural scaffolds, followed by a process known as protein inverse folding, which involves sequence inference on these scaffolds. However, these methodologies face significant challenges when applied to hyper-variable structures such as antibody Complementarity-Determining Regions (CDRs), where sequence inference frequently results in non-functional sequences due to hallucinations. Distinguished from prevailing protein inverse folding approaches, this paper introduces Igseek, a novel structure-retrieval framework that infers CDR sequences by retrieving similar structures from a natural antibody database. Specifically, Igseek employs a simple yet effective multi-channel equivariant graph neural network to generate high-quality geometric representations of CDR backbone structures. Subsequently, it aligns sequences of structurally similar CDRs and utilizes structurally conserved sequence motifs to enhance inference accuracy. Our experiments demonstrate that Igseek not only proves to be highly efficient in structural retrieval but also outperforms state-of-the-art approaches in sequence recovery for both antibodies and T-Cell Receptors, offering a new retrieval-based perspective for therapeutic protein design.

q-bio.BM↗

arXiv2016

AptRank: An Adaptive PageRank Model for Protein Function Prediction on Bi-relational Graphs

Diffusion-based network models are widely used for protein function prediction using protein network data and have been shown to outperform neighborhood- and module-based methods. Recent studies have shown that integrating the hierarchical structure of the Gene Ontology (GO) data dramatically improves prediction accuracy. However, previous methods usually either used the GO hierarchy to refine the prediction results of multiple classifiers, or flattened the hierarchy into a function-function similarity kernel. No study has taken the GO hierarchy into account together with the protein network as a two-layer network model. We first construct a Bi-relational graph (Birg) model comprised of both protein-protein association and function-function hierarchical networks. We then propose two diffusion-based methods, BirgRank and AptRank, both of which use PageRank to diffuse information on this two-layer graph model. BirgRank is an application of traditional PageRank with fixed decay parameters. In contrast, AptRank uses an adaptive mechanism to improve the performance of BirgRank. We evaluate both methods in predicting protein function on yeast, fly, and human datasets, and compare with four previous methods: GeneMANIA, TMC, ProteinRank and clusDCA. We design three validation strategies: missing function prediction, de novo function prediction, and guided function prediction to comprehensively evaluate all six methods. We find that both BirgRank and AptRank outperform the others, especially in missing function prediction when using only 10% of the data for training. AptRank combines protein-protein associations and the GO function-function hierarchy into a two-layer network model without flattening the hierarchy into a similarity kernel. Introducing an adaptive mechanism to the traditional, fixed-parameter model of PageRank greatly improves the accuracy of protein function prediction.

q-bio.MN↗