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Casper Kunstmann-Olsen

Publications and source records attributed to Casper Kunstmann-Olsen.

2 recordsLinked to original sources

Non-Destructive Prediction of Fruit Ripeness and Firmness Using Hyperspectral Imaging and Lightweight Machine Learning Models

Post-harvest fruit quality assessment is essential for reducing food waste, yet reliable non-destructive methods typically depend on expensive hyperspectral cameras and computationally intensive deep learning models. These systems typically require GPU resources, large-scale training data, and domain expertise, limiting their feasibility for many real-world agricultural settings. This study systematically evaluates 20 classical machine learning algorithms on hyperspectral imaging data for simultaneous ripeness classification and firmness prediction across five fruit species, using cross-validated experimental design with Bayesian hyperparameter optimization. Data preprocessing strategy, particularly class balancing and spectral transformations, contributes as much to prediction accuracy as algorithm choice. Our results show that tree-based machine learning models can outperform state-of-the-art deep earning models reported in Fruit-HSNet. Moreover, the findings indicate that only three visible-range wavelengths are needed to recover over 94% of full-spectrum accuracy, demonstrating that low-cost multispectral sensors combined with lightweight machine learning models can serve as practical alternatives to expensive hyperspectral cameras and complex deep learning approaches for practical fruit quality sorting.

eess.IV

Local sensing of absolute refractive index during protein-binding using microlasers with spectral encoding

Multiplexed, specific and sensitive detection of antigens is critical for the rapid and accurate diagnosis of disease and the informed development of personalized treatment plans. Here, we show that polymer microsphere lasers can be used as photonic sensors to monitor and quantify direct surface binding of biomolecules via changes in the refractive index. The unique spectral signature of each individual laser can be used to find their size and effective refractive index which adds a new encoding dimension when compared to conventional fluorescent beads. We utilize antibody-functionalized microlasers to selectively detect protein binding. Different stages of the multilayer surface modification can be resolved, and protein binding is demonstrated for two different proteins, IgG and CRP. Moreover, by continuously monitoring single lasers, we demonstrate the possibility of real-time monitoring of binding dynamics between antigens in solution phase and the immobilized antibodies. For multiplexed detection, the microlasers are employed in a flow cytometer configuration, with fast spectral detection and identification of microlasers with and without antigen binding. We envision that by combining microlasers with well-established surface modification chemistries and flow geometries, the multiplexing ability of microbead immunoassays can be strongly increased while also opening avenues for single cell profiling within heterogenous cell populations.

physics.optics