SearcharxivSearch

arXiv subjects

Chao Zuo

Publications and source records attributed to Chao Zuo.

At least 19 recordsLinked to original sources

QdaVPR: A novel query-based domain-agnostic model for visual place recognition

Visual place recognition (VPR) aiming at predicting the location of an image based solely on its visual features is a fundamental task in robotics and autonomous systems. Domain variation remains one of the main challenges in VPR and is relatively unexplored. Existing VPR models attempt to achieve domain agnosticism either by training on large-scale datasets that inherently contain some domain variations, or by being specifically adapted to particular target domains. In practice, the former lacks explicit domain supervision, while the latter generalizes poorly to unseen domain shifts. This paper proposes a novel query-based domain-agnostic VPR model called QdaVPR. First, a dual-level adversarial learning framework is designed to encourage domain invariance for both the query features forming the global descriptor and the image features from which these query features are derived. Then, a triplet supervision based on query combinations is designed to enhance the discriminative power of the global descriptors. To support the learning process, we augment a large-scale VPR dataset using style transfer methods, generating various synthetic domains with corresponding domain labels as auxiliary supervision. Extensive experiments show that QdaVPR achieves state-of-the-art performance on multiple VPR benchmarks with significant domain variations. Specifically, it attains the best Recall@1 and Recall@10 on nearly all test scenarios: 93.5%/98.6% on Nordland (seasonal changes), 97.5%/99.0% on Tokyo24/7 (day-night transitions), and the highest Recall@1 across almost all weather conditions on the SVOX dataset. Our code will be released at https://github.com/shuimushan/QdaVPR.

cs.CV

Simultaneous super-resolution and optical sectioning with four-beam interference structured illumination microscopy (4I-SIM)

Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when applied to thick or scattering specimens, conventional two-dimensional SIM (2D-SIM) suffers from the missing cone problem in its optical transfer function, resulting in prominent out-of-focus background and severe reconstruction artifacts that compromise image fidelity. Here, we present four-beam interference structured illumination microscopy (4I-SIM), which introduces additional interference orders to expand lateral frequency support and compensate the axial missing cone simultaneously. This strategy achieves artifact-free super-resolution with intrinsic optical sectioning, effectively overcoming the fundamental limitation of 2D-SIM without additional acquisition overhead. Experimental validation across diverse thick fixed and live specimens demonstrates that 4I-SIM delivers nearly twofold lateral resolution enhancement and substantially improved sectioning compared with its 2D counterpart, achieving lateral and axial resolutions of 103 nm and 336 nm, respectively. In particular, 4I-SIM reveals mitochondrial remodeling and apoptosis under high-glucose stress with millisecond temporal resolution -- features that remain obscured with conventional SIM. With minimal hardware modification, low phototoxicity, and open-source reconstruction tools, 4I-SIM establishes a practical and reproducible platform for simultaneous super-resolution and optical sectioning imaging in complex biological environments.

eess.IV

Doubling the field of view and eliminating the replica overlap problem in common-path shearing quantitative phase imaging

Quantitative phase imaging (QPI) enables label-free, high-contrast visualization of transparent specimens, but its common implementation in off-axis digital holographic microscopy (DHM) requires a separate reference beam, which increases system complexity and sensitivity to noise and vibrations. Common-path shearing DHMs overcome these drawbacks by eliminating the reference arm, yet they suffer from sheared object beam (replica) overlap, as both interfering sheared beams traverse the sample and generate superimposed phase images. This limits their use to sparse objects only. Here we introduce R2D-QPI, a method that numerically decouples object and replica fields of view through controlled shear scanning. The method analytically separates overlapped phase images and effectively doubles the imaged area, requiring only two measurements. We experimentally validate the approach on a phase resolution test target, yeast cells, and human thyroid tissue slices, demonstrating accurate reconstruction even in highly confluent samples with strong object-replica overlap. The results establish R2D-QPI as a robust and versatile solution for common-path QPI, enabling wide-field, label-free phase imaging with minimal data acquisition and strong potential for applications in biological and medical microscopy.

physics.optics

Fourier ptychographic microscopy aided with transport of intensity equation for robust full phase spectrum reconstruction

Fourier ptychographic microscopy (FPM) is a pivotal computational imaging technique that achieves phase and amplitude reconstruction with high resolution and wide field of view, using low numerical aperture objectives and LED array illumination. Despite its unique strengths, FPM remains fundamentally limited in retrieving low spatial frequency phase information due to the absence of phase encoding in all brightfield illumination angles. To overcome this, we present a novel hybrid approach that combines FPM with the transport of intensity equation (TIE), enabling accurate, full-spectrum phase retrieval without compromising system simplicity. Our method extends standard FPM acquisitions with a single additional on-axis defocused image, from which low-frequency phase components are reconstructed via TIE method, employing large defocus distance to suppress low-frequency artifacts and enhance robustness to intensity noise. To additionally compensate for defocus-induced magnification variations caused by spherical wavefront illumination, we employ an affine transform-based correction scheme upon image registration. Notably, by restoring the missing low-frequency content, our hybrid method appears capable of recovering phase values beyond the conventional 0-2{\pi} range - an area where conventional FPM techniques often struggle when dealing with optically thick samples. We validated our method using a quantitative phase test target for benchmarking accuracy and biological cheek cells, mouse neurons, and mouse brain tissue slice samples to demonstrate applicability for in vitro bioimaging. Experimental results confirm substantial improvements in phase reconstruction fidelity across spatial frequencies, establishing this hybrid FPM+TIE framework as a practical and high-performance solution for quantitative phase imaging in biomedical and optical metrology applications.

physics.optics

Energy-resolved polarisation study of the Crab Nebula with IXPE

This work presents a new detailed study on the energy-dependent variation in the X-ray polarisation of the Crab Pulsar Wind Nebula (PWN), obtained using data from the Imaging X-ray Polarimetry Explorer (IXPE). For the entire PWN, we observed a linear variation in polarisation degree (PD), and detected the rotation of the polarisation angle (PA) with the energy at higher than 99.9999\% of the confidence level. This energy-dependent polarisation variation is in line with the indication found in Vela PWN by IXPE, and it can be interpreted as the emitting region of the polarised photons shrinks with increasing energy, leading to higher PD because they are less influenced by the turbulence of the magnetic field. We compared the IXPE polarisation results with those of other hard X-ray/gamma observatories (PoGO+, Intregral, AstroSat) for the PWN, finding the same trend from soft-X to hard-X with the PD increasing with the energy and the PA approaching the pulsar's spin axis. In fact, in this wide energy band, the fitting results show an energy trend for the PA compatible with the estimated pulsar's spin axis within 3$\sigma$ of confidence level.

astro-ph.HE

Spatially resolved polarization variation of the Crab Nebula

We examined the spatially resolved polarization variations in the Crab Nebula over 2 yr, using observational data from the Imaging X-ray Polarimetry Explorer, and offer key insights into its magnetic field structures and evolution. The results show significant temporal changes in the polarization degree (PD) across three regions of interest in the 2-8 keV energy band. Regions (a) and (b), located in the northern and the southwestern parts of the study area, exhibit PD variations with significance levels greater than 4 sigma and 3 sigma , respectively. Region (c), located in the southwest,shows a notable decrease in PD with a significance greater than 5 sigma. However, no significant variation in the polarization angle was observed. Meanwhile, notable flux variations were detected, likely influenced by dynamic processes such as magnetized turbulence within the nebula.

astro-ph.HE

Differentiable Imaging Meets Adaptive Neural Dropout: An Advancing Method for Transparent Object Tomography

Label-free tomographic microscopy offers a compelling means to visualize three-dimensional (3D) refractive index (RI) distributions from two-dimensional (2D) intensity measurements. However, limited forward-model accuracy and the ill-posed nature of the inverse problem hamper artifact-free reconstructions. Meanwhile, artificial neural networks excel at modeling nonlinearities. Here, we employ a Differentiable Imaging framework that represents the 3D sample as a multi-layer neural network embedding physical constraints of light propagation. Building on this formulation, we propose a physics-guided Adaptive Dropout Neural Network (ADNN) for optical diffraction tomography (ODT), focusing on network topology and voxel-wise RI fidelity rather than solely on input-output mappings. By exploiting prior knowledge of the sample's RI, the ADNN adaptively drops and reactivates neurons, enhancing reconstruction accuracy and stability. We validate this method with extensive simulations and experiments on weakly and multiple-scattering samples under different imaging setups. The ADNN significantly improves quantitative 3D RI reconstructions, providing superior optical-sectioning and effectively suppressing artifacts. Experimental results show that the ADNN reduces the Mean Absolute Error (MAE) by a factor of 3 to 5 and increases the Structural Similarity Index Metric (SSIM) by about 4 to 30 times compared to the state-of-the-art approach.

physics.optics

Gradient Optical Diffraction Tomography

Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials sciences, and other fields. ODT bypasses the inherent limitations of 2D integrated quantitative phase imaging methods. To increase the signal-to-noise ratio easy-to-implement common-path shearing interferometry setups are successfully combined with low spatiotemporal coherence of illumination. The need for self-interference generated holograms, with small shear values, critically impedes the analysis of dense and thick samples, e.g., cell cultures, tissue sections, and embryos/organoids. Phase gradient imaging techniques, deployed as a popular solution in the small shear regime, up to now were constrained to 2D integrated quasi-quantitative phase imaging and z-scanning for depth resolution. To fill this significant scientific gap, we propose a novel gradient optical diffraction tomography (GODT) method. The GODT uses coherence-tailored illumination-scanning sequence of phase gradient measurements to tomographically reconstruct, for the first time to the best of our knowledge, a derivative of the 3D RI distribution (in the shear direction) with clearly visible 3D sample structure and high sensitivity to its spatial variations. We present a mathematically rigorous theory based on the first-order Rytov approximation behind the new method, validate it using simulations deploying numerical Shepp-Logan target and corroborate experimentally via successful tomographic imaging of the calibrated nano-printed cell phantom and efficient examination of neural cells. This novel imaging modality opens new possibilities in biomedical quantitative phase imaging, advancing the field and putting forward a first of a kind contrast domain: 3D RI gradient.

physics.optics

Highly Significant Detection of X-Ray Polarization from the Brightest Accreting Neutron Star Sco X-1

The Imaging X-ray Polarimetry Explorer (IXPE) measured with high significance the X-ray polarization of the brightest Z-source Scorpius X-1, resulting in the nominal 2-8 keV energy band in a polarization degree of 1.0(0.2)% and a polarization angle of 8(6)° at 90% of confidence level. This observation was strictly simultaneous with observations performed by NICER, NuSTAR, and Insight-HXMT, which allowed for a precise characterization of its broad-band spectrum from soft to hard X-rays. The source has been observed mainly in its soft state, with short periods of flaring. We also observed low-frequency quasi-periodic oscillations. From a spectro-polarimetric analysis, we associate a polarization to the accretion disk at <3.2% at 90% of confidence level, compatible with expectations for an electron-scattering dominated optically thick atmosphere at the Sco X-1 inclination of 44°; for the higher-energy Comptonized component, we obtain a polarization of 1.3(0.4)%, in agreement with expectations for a slab of Thomson optical depth of ~7 and an electron temperature of ~3 keV. A polarization rotation with respect to previous observations by OSO-8 and PolarLight, and also with respect to the radio-jet position angle, is observed. This result may indicate a variation of the polarization with the source state that can be related to relativistic precession or to a change in the corona geometry with the accretion flow.

astro-ph.HE

Physics-driven universal twin-image removal network for digital in-line holographic microscopy

Digital in-line holographic microscopy (DIHM) enables efficient and cost-effective computational quantitative phase imaging with a large field of view, making it valuable for studying cell motility, migration, and bio-microfluidics. However, the quality of DIHM reconstructions is compromised by twin-image noise, posing a significant challenge. Conventional methods for mitigating this noise involve complex hardware setups or time-consuming algorithms with often limited effectiveness. In this work, we propose UTIRnet, a deep learning solution for fast, robust, and universally applicable twin-image suppression, trained exclusively on numerically generated datasets. The availability of open-source UTIRnet codes facilitates its implementation in various DIHM systems without the need for extensive experimental training data. Notably, our network ensures the consistency of reconstruction results with input holograms, imparting a physics-based foundation and enhancing reliability compared to conventional deep learning approaches. Experimental verification was conducted among others on live neural glial cell culture migration sensing, which is crucial for neurodegenerative disease research.

eess.IV

Generation of S-shaped photonic hooks from microcylinders with engineered surface patches

Photonic hooks (PHs) are non-evanescent light beams with a highly concentrated curved optical fields. Since their discovery, PHs always have one single inflection point and thus have a hook-like structure. In this work, a new type of PHs with two inflection points and S-shaped structures (S-PHs) were reported for the first time. We theoretically studied the effects of various physical parameters on the generation of S-PHs. Furthermore, we showed that decorating particles with multiple patches can significantly enhance the curvature and length of the S-PHs. The S-PHs may have potential applications in super-resolution imaging, sub-wavelength micromachining, particle and cell manipulation, etc.

physics.optics

Generation of photonic hooks from patchy microcylinders

The photonic hook (PH) is new type of curved light beam which has promising applications in various fields such as nanoparticle manipulation, super-resolution imaging, etc. Herein, we proposed a new approach of utilizing patchy microcylinders for the generation of PHs. Numerical simulation based on the finite-difference time-domain method was used to investigate the field distribution characteristics of the PHs. By rotating the patchy microcylinder, PHs with different curvatures can be effectively generated, and the PH with a bending angle of 28.4$^\circ$ and a full-width-half-maximum of 0.36 $λ$ can be obtained from 1 $μ$m-diameter patchy microcylinders.

physics.optics

Super-resolution imaging with patchy microspheres

The diffraction limit is a fundamental barrier in optical microscopy, which restricts the smallest resolvable feature size of a microscopic system. Microsphere-based microscopy has proven to be a promosing tool for challenging the diffraction limit. Nevertheless, the microspheres have a low imaging contrast in the air, which hinders the application of this technique. In this Letter, we demonstrate that this challenge can be effectively overcome by using partially Ag-plated microspheres. The deposited Ag film acts as an aperture stop that blocks a portion of the incident beam, forming a photonic hook with oblique near-field illumination. Such a photonic hook significantly enhanced imaging contrast, as experimentally verified by imaging Blu-ray disc surface and silica particle arrays.

physics.optics

Deep-learning-enabled geometric constraints and phase unwrapping for single-shot absolute 3D shape measurement

Fringe projection profilometry (FPP) is one of the most popular three-dimensional (3D) shape measurement techniques, and has becoming more prevalently adopted in intelligent manufacturing, defect detection and some other important applications. In FPP, how to efficiently recover the absolute phase has always been a great challenge. The stereo phase unwrapping (SPU) technologies based on geometric constraints can eliminate phase ambiguity without projecting any additional fringe patterns, which maximizes the efficiency of the retrieval of absolute phase. Inspired by the recent success of deep learning technologies for phase analysis, we demonstrate that deep learning can be an effective tool that organically unifies the phase retrieval, geometric constraints, and phase unwrapping steps into a comprehensive framework. Driven by extensive training dataset, the neutral network can gradually "learn" how to transfer one high-frequency fringe pattern into the "physically meaningful", and "most likely" absolute phase, instead of "step by step" as in convention approaches. Based on the properly trained framework, high-quality phase retrieval and robust phase ambiguity removal can be achieved based on only single-frame projection. Experimental results demonstrate that compared with traditional SPU, our method can more efficiently and stably unwrap the phase of dense fringe images in a larger measurement volume with fewer camera views. Limitations about the proposed approach are also discussed. We believe the proposed approach represents an important step forward in high-speed, high-accuracy, motion-artifacts-free absolute 3D shape measurement for complicated object from a single fringe pattern.

eess.IV

On a universal solution to the transport-of-intensity equation

Transport-of-intensity equation (TIE) is one of the most well-known approaches for phase retrieval and quantitative phase imaging. It directly recovers the quantitative phase distribution of an optical field by through-focus intensity measurements in a noninterferometic, deterministic manner. Nevertheless, the accuracy and validity of state-of-the-art TIE solvers depend on restrictive preknowledge or assumptions, including appropriate boundary conditions, a well-defined closed region, and quasi-uniform in-focus intensity distribution, which, however, cannot be strictly satisfied simultaneously under practical experimental conditions. In this Letter, we propose a universal solution to TIE with the advantages of high accuracy, convergence guarantee, applicability to arbitrarily-shaped regions, and simplified implementation and computation. With the "maximum intensity assumption", we firstly simplified TIE as a standard Possion equation to get an initial guess of the solution. Then the initial solution is further refined iteratively by solving the same Possion equation, and thus, the instability associated with the division by zero/small intensity values and large intensity variations can be effectively bypassed. Simulations and experiments with arbitrary phase, arbitrary aperture shapes, and nonuniform intensity distributions verify the effectiveness and universality of the proposed method.

eess.IV

High-speed in vitro intensity diffraction tomography

We demonstrate a label-free, scan-free {\it intensity} diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume 3D refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60$\times$ to achieve high-speed 10 Hz volume rates. Using 8 intensity images, we recover $\sim350\times100\times20μ$m$^3$ volumes with near diffraction-limited lateral resolution of 487 nm and axial resolution of 3.4 $μ$m. Our technique's large volume rate and high resolution enables 3D quantitative phase imaging of complex living biological samples across multiple length scales. We demonstrate aIDT's capabilities on unicellular diatom microalgae, epithelial buccal cell clusters with native bacteria, and live \emph{Caenorhabditis elegans} specimens. Within these samples, we recover macro-scale cellular structures, subcellular organelles, and dynamic micro-organism tissues with minimal motion artifacts. Quantifying such features has significant utility in oncology, immunology, and cellular pathophysiology, where these morphological features are evaluated for changes in the presence of disease, parasites, and new drug treatments. Finally, we simulate our aIDT system to highlight the accuracy and sensitivity of our technique. aIDT shows promise as a powerful high-speed, label-free computational microscopy technique applications where natural imaging is required to evaluate environmental effects on a sample in real-time. We provide example datasets and an open source implementation of aIDT at \href{https://github.com/bu-cisl/IDT-using-Annular-Illumination}{https://github.com/bu-cisl/IDT-using-Annular-Illumination}.

physics.optics

Resolution analysis in a lens-free on-chip digital holographic microscope

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it. The scattered object wave interferes with the reference (unscattered) wave at the plane where a digital sensor is situated, producing a digital hologram that can be processed in several ways to extract and numerically reconstruct an in-focus image using the back propagation algorithm. Without requiring any lenses and other intermediate optical components, the LFOCDHM has unique advantages of offering a large effective numerical aperture (NA) close to unity across the native wide field-of-view (FOV) of the imaging sensor in a cost-effective and compact design. However, unlike conventional coherent diffraction limited imaging systems, where the limiting aperture is used to define the system performance, typical lens-free microscopes only produce compromised imaging resolution that far below the ideal coherent diffraction limit. At least five major factors may contribute to this limitation, namely, the sample-to-sensor distance, spatial and temporal coherence of the illumination, finite size of the equally spaced sensor pixels, and finite extent of the image sub-FOV used for the reconstruction, which have not been systematically and rigorously explored until now. In this work, we derive five transfer function models that account for all these physical effects and interactions of these models on the imaging resolution of LFOCDHM. We also examine how our theoretical models can be utilized to optimize the optical design or predict the theoretical resolution limit of a given LFOCDHM system. We present a series of simulations and experiments to confirm the validity of our theoretical models.

physics.optics

Wide-field high-resolution 3D microscopy with Fourier ptychographic diffraction tomography

We report a computational 3D microscopy technique, termed Fourier ptychographic diffraction tomography (FPDT), that iteratively stitches together numerous variably illuminated, low-resolution images acquired with a low-numerical aperture (NA) objective in 3D Fourier space to create a wide field-of-view (FOV), high-resolution, depth-resolved complex refractive index (RI) image across large volumes. Unlike conventional optical diffraction tomography (ODT) approaches that rely on controlled bright-field illumination, holographic phase measurement, and high-NA objective detection, FPDT employs tomographic RI reconstruction from low-NA intensity-only measurements. In addition, FPDT incorporates high-angle dark-field illuminations beyond the NA of the objective, significantly expanding the accessible object frequency. With FPDT, we present the highest-throughput ODT results with 390nm lateral resolution and 899nm axial resolution across a 10X FOV of 1.77mm2 and a depth of focus of ~20μm. Billion-voxel 3D tomographic imaging results of biological samples establish FPDT as a powerful non-invasive and label-free tool for high-throughput 3D microscopy applications.

physics.optics