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E. Gladilin

Publications and source records attributed to E. Gladilin.

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Contactless estimation of continuum displacement and mechanical compressibility from image series using a deep learning based framework

Contactless and non-invasive estimation of mechanical properties of physical media from optical observations is of interest for manifold engineering and biomedical applications, where direct physical measurements are not possible. Conventional approaches to the assessment of image displacement and non-contact material probing typically rely on time-consuming iterative algorithms for non-rigid image registration and constitutive modelling using discretization and iterative numerical solving techniques, such as Finite Element Method (FEM) and Finite Difference Method (FDM), which are not suitable for high-throughput data processing. Here, we present an efficient deep learning based end-to-end approach for the estimation of continuum displacement and material compressibility directly from the image series. Based on two deep neural networks for image registration and material compressibility estimation, this framework outperforms conventional approaches in terms of efficiency and accuracy. In particular, our experimental results show that the deep learning model trained on a set of reference data can accurately determine the material compressibility even in the presence of substantial local deviations of the mapping predicted by image registration from the reference displacement field. Our findings suggest that the remarkable accuracy of the deep learning end-to-end model originates from its ability to assess higher-order cognitive features, such as the vorticity of the vector field, rather than conventional local features of the image displacement.

cs.CV

The role of vimentin in regulating cell-invasive migration in dense cultures of breast carcinoma cells

Cell migration and mechanics are tightly regulated by the integrated activities of the various cytoskeletal networks. In cancer cells, cytoskeletal modulations have been implicated in the loss of tissue integrity, and acquisition of an invasive phenotype. In epithelial cancers, for example, increased expression of the cytoskeletal filament protein vimentin correlates with metastatic potential. Nonetheless, the exact mechanism whereby vimentin affects cell motility remains poorly understood. In this study, we measured the effects of vimentin expression on the mechano-elastic and migratory properties of the highly invasive breast carcinoma cell line MDA231. We demonstrate here that vimentin stiffens cells and enhances cell migration in dense cultures, but exerts little or no effect on the migration of sparsely plated cells. These results suggest that cell-cell interactions play a key role in regulating cell migration, and coordinating cell movement in dense cultures. Our findings pave the way towards understanding the relationship between cell migration and mechanics, in a biologically relevant context.

physics.bio-ph