DINO-Nucleus: Multi-Level Self-Supervised Features for Nucleus Instance Segmentation
Nucleus instance segmentation and counting are essential for quantitative microscopy, enabling reliable analysis of cell populations, tissue organization, and disease-related changes. However, accurately separating touching nuclei remains difficult, and many existing approaches rely on complex models or sensitive post-processing procedures. We present DINO-Nucleus, a parameter-efficient framework that leverages multi-level self-supervised DINOv2 representations for nucleus instance segmentation and counting. The model jointly predicts nucleus regions, pixel-wise instance flows, and explicit nucleus centers, while a consistency objective encourages the predicted flows to converge toward their corresponding centers. Multi-level transformer features provide complementary spatial and semantic information, and the pretrained backbone is adapted using lightweight LoRA modules to reduce fine-tuning cost. Experimental results show that DINO-Nucleus achieves strong segmentation and counting performance with parameter-efficient backbone adaptation. These findings demonstrate that parameter-efficient adaptation of multi-level self-supervised features can provide an effective solution for nucleus instance segmentation in microscopy images.