arXiv subjects

Emmanuel Bossy

Publications and source records attributed to Emmanuel Bossy.

27 records · Page 2Linked to original sources

arXiv2015

Single-side access, isotropic resolution and multispectral 3D photoacoustic imaging with rotate-translate scanning of ultrasonic detector array

Photoacoustic imaging can achieve high-resolution three-dimensional visualization of optical absorbers at penetration depths ~ 1 cm in biological tissues by detecting optically-induced high ultrasound frequencies. Tomographic acquisition with ultrasound linear arrays offers an easy implementation of single-side access, parallelized and high-frequency detection, but usually comes with an image quality impaired by the directionality of the detectors. Indeed, a simple translation of the array perpendicularly to its median imaging plane is often used, but results both in a poor resolution in the translation direction and in strong limited view artifacts. To improve the spatial resolution and the visibility of complex structures while keeping a planar detection geometry, we introduce, in this paper, a novel rotate-translate scanning scheme, and investigate the performance of a scanner implemented at 15 MHz center frequency. The developed system achieved a quasi-isotropic uniform 3D resolution of ~170 um over a cubic volume of side length 8.5 mm, i.e. an improvement in the resolution in the translation direction by almost one order of magnitude. Dual wavelength imaging was also demonstrated with ultrafast wavelength shifting. The validity of our approach was shown in vitro. We discuss the ability to enable in vivo imaging for preclinical and clinical studies.

physics.optics↗

arXiv2015

Optical-resolution photoacoustic imaging through thick tissue with a thin capillary as a dual optical-in acoustic-out waveguide

We demonstrate the ability to guide high-frequency photoacoustic waves through thick tissue with a water-filled silica-capillary (150 μm inner diameter and 30 mm long). An optical-resolution photoacoustic image of a 30 μm diameter absorbing nylon thread was obtained by guiding the acoustic waves in the capillary through a 3 cm thick fat layer. The transmission loss through the capillary was about -20 dB, much lower than the -120 dB acoustic attenuation through the fat layer. The overwhelming acoustic attenuation of high-frequency acoustic waves by biological tissue can therefore be avoided by the use of a small footprint capillary acoustic waveguide for remote detection. We finally demonstrate that the capillary can be used as a dual optical-in acoustic-out waveguide, paving the way for the development of minimally invasive optical-resolution photoacoustic endoscopes free of any acoustic or optical elements at their imaging tip.

physics.optics↗

arXiv2014

Light Focusing and Two-Dimensional Imaging Through Scattering Media using the Photoacoustic Transmission-Matrix with an Ultrasound Array

We implement the photoacoustic transmission-matrix approach on a two-dimensional photoacoustic imaging system, using a 15 MHz linear ultrasound array. Using a black leaf skeleton as a complex absorbing structure, we demonstrate that the photoacoustic transmission-matrix approach allows to reveal structural features that are invisible in conventional photoacoustic images, as well as to selectively control light focusing on absorbing targets, leading to a local enhancement of the photoacoustic signal.

physics.optics↗

arXiv2013

Improving visibility in photoacoustic imaging using dynamic speckle illumination

In high-frequency photoacoustic imaging with uniform illumination, homogeneous photo-absorbing structures may be invisible because of their large size or limited-view issues. Here we show that, by exploiting dynamic speckle illumination, it is possible to reveal features which are normally invisible with a photoacoustic system comprised of a 20MHz linear ultrasound array. We demonstrate imaging of a 5 mm diameter absorbing cylinder and a 30 micrometer black thread arranged in a complex shape. The hidden structures are directly retrieved from photoacoustic images recorded for different random speckle illuminations of the phantoms by assessing the variation in the value of each pixel over the illumination patterns.

physics.optics↗

arXiv2013

Improving Photoacoustic-guided Focusing in Scattering Media by Spectrally Filtered Detection

We experimentally and numerically study the potential of photoacoustic-guiding for light focusing through scattering samples via wavefront-shaping and iterative optimization. We experimentally demonstrate that the focusing efficiency on an extended absorber can be improved by iterative optimization of the high frequency components of the broadband photoacoustic signal detected with a spherically focused transducer. We demonstrate more than 8-fold increase in the photoacoustic signal generated by a 30 microns wire using a narrow frequency band around 60MHz. We numerically confirm that such optimization leads to a smaller optical focus than using the low frequency content of the photoacoustic feedback.

physics.optics↗

arXiv2013

Influence of nanoscale temperature rises on photoacoustic generation: discrimination between optical absorbers based on nonlinear photoacoustics at high frequency

In the thermoelastic regime, photoacoustic sensing of optical absorption relies on conversion from light to acoustic energy via the coefficient of thermal expansion β. In this work, we confront confront experimental measurements to theoretical predictions of nonlinear photoacoustic generation based on the dynamic variation of β(T) during the optical excitation of absorbers in aqueous solution. The photoacoustic generation from solutions of organic dye and gold nanospheres (with same optical densities), illuminated with 532 nm nanosecond pulses, was detected using a high frequency ultrasound transducer (center frequency 20 MHz). Photoacoustic emission was observed with gold nanospheres at low fluence (a few mJ/cm2) for an equilibrium temperature around 4°C, where the linear photoacoustic effect in water vanishes, highlighting the nonlinear emission from the solution of nanospheres. Under the same condition, no emission was observed with the absorbing organic dye. At a fixed fluence of 5 mJ/cm2, the photoacoustic amplitude was studied as a function of the equilibrium temperature from 2°C to 20°C. While the photoacoustic amplitude from the organic dye followed β(T) in water and vanished around 4°C, the photoacoustic amplitude from the gold nanospheres remained significant over the whole temperature range. These experimental results are shown to be in qualitative agreement with theoretical calculations based on the nanoscale and nanosecond transient changes of the coefficient of thermal expansion of water around the gold nanospheres. Our results suggest that in the context of high frequency photoacoustic imaging, nanoparticles may be discriminated from molecular absorbers based on either the nonlinear fluence-dependence or the equilibrium temperature-dependence of the photoacoustic amplitude.

physics.optics↗

arXiv2013

Radiative transfer and diffusion limits for wave field correlations in locally shifted random media

The aim of this paper is to develop a mathematical framework for opto-elastography. In opto-elastography, a mechanical perturbation of the medium produces a decorrelation of optical speckle patterns due to the displacements of optical scatterers. To model this, we consider two optically random media, with the second medium obtained by shifting the first medium in some local region. We derive the radiative transfer equation for the cross-correlation of the wave fields in the media. Then we derive its diffusion approximation. In both the radiative transfer and the diffusion regimes, we relate the correlation of speckle patterns to the solutions of the radiative transfer and the diffusion equations. We present numerical simulations based on our model which are in agreement with recent experimental measurements.

math-ph↗

arXiv2013

Optical-resolution photoacoustic microscopy by use of a multimode fiber

We demonstrate Optical-Resolution Photoacoustic Microscopy (OR-PAM), where the optical field is focused and scanned using Digital Phase Conjugation (DPC) through a multimode fiber. The focus is scanned across the field of view using digital means, and the acoustic signal induced is collected by a transducer. Optical-resolution photoacoustic images of a knot made by two absorptive wires are obtained and we report on resolution smaller than 1.5μm across a 201μm by 201μm field of view. The use of a multimode optical fiber for the optical excitation part can pave the way for miniature endoscopes that can provide optical-resolution photoacoustic images at large optical depth.

physics.optics↗

arXiv2012

Acousto-optical coherence tomography with a digital holographic detection scheme

Acousto-optical coherence tomography (AOCT) consists in using random phase jumps on ultrasound and light to achieve a millimeter resolution when imaging thick scattering media. We combined this technique with heterodyne off-axis digital holography. Two-dimensional images of absorbing objects embedded in scattering phantoms are obtained with a good signal-to-noise ratio. We study the impact of the phase modulation characteristics on the amplitude of the acousto-optic signal and on the contrast and apparent size of the absorbing inclusion.

physics.optics↗