Fabio Naro | Searcharxiv

arXiv2019

Modulation of the cardiomyocyte contraction inside a hydrostatic pressure bioreactor: $\textit{in vitro}$ verification of the Frank-Starling law

We have studied beating mouse cardiac syncytia $\textit{in vitro}$ in order to assess the inotropic, ergotropic, and chronotropic effects of both increasing and decreasing hydrostatic pressures. In particular, we have performed an image processing analysis to evaluate the kinematics and the dynamics of those pressure-loaded beating syncytia starting from the video registration of their contraction movement. By this analysis, we have verified the Frank-Starling law of the heart in $\textit{in vitro}$ beating cardiac syncytia and we have obtained their geometrical-functional classification.

q-bio.OT↗

arXiv2019

Field models and numerical dosimetry inside an extremely-low-frequency electromagnetic bioreactor: the theoretical link between the electromagnetically induced mechanical forces and the biological mechanisms of the cell tensegrity

We have implemented field models and performed a detailed numerical dosimetry inside our extremely-low-frequency electromagnetic bioreactor which has been successfully used in $\textit{in vitro}$ Biotechnology and Tissue Engineering researches. The numerical dosimetry permitted to map the magnetic induction field (maximum module equal to about 3.3 mT) and to discuss its biological effects in terms of induced electric currents and induced mechanical forces (compression and traction). So, in the frame of the tensegrity-mechanotransduction theory of Ingber, the study of these electromagnetically induced mechanical forces could be, in our opinion, a powerful tool to understand some effects of the electromagnetic stimulation whose mechanisms remain still elusive.

q-bio.OT↗