arXiv subjects

Francisca F. Fernandes

Publications and source records attributed to Francisca F. Fernandes.

4 recordsLinked to original sources

arXiv2022

MP-PCA denoising of fMRI time-series data can lead to artificial activation "spreading"

MP-PCA denoising has become the method of choice for denoising in MRI since it provides an objective threshold to separate the desired signal from unwanted thermal noise components. In rodents, thermal noise in the coils is an important source of noise that can reduce the accuracy of activation mapping in fMRI. Further confounding this problem, vendor data often contains zero-filling and other effects that may violate MP-PCA assumptions. Here, we develop an approach to denoise vendor data and assess activation "spreading" caused by MP-PCA denoising in rodent task-based fMRI data. Data was obtained from N = 3 mice using conventional multislice and ultrafast acquisitions (1 s and 50 ms temporal resolution, respectively), during visual stimulation. MP-PCA denoising produced SNR gains of 64% and 39% and Fourier spectral amplitude (FSA) increases in BOLD maps of 9% and 7% for multislice and ultrafast data, respectively, when using a small [2 2] denoising window. Larger windows provided higher SNR and FSA gains with increased spatial extent of activation that may or may not represent real activation. Simulations showed that MP-PCA denoising causes activation "spreading" with an increase in false positive rate and smoother functional maps due to local "bleeding" of principal components, and that the optimal denoising window for improved specificity of functional mapping, based on Dice score calculations, depends on the data's tSNR and functional CNR. This "spreading" effect applies also to another recently proposed low-rank denoising method (NORDIC). Our results bode well for dramatically enhancing spatial and/or temporal resolution in future fMRI work, while taking into account the sensitivity/specificity trade-offs of low-rank denoising methods.

physics.med-ph↗

arXiv2021

Effective bowel motion reduction in mouse abdominal MRI using hyoscine butylbromide

Purpose: Bowel motion is a significant source of artifacts in mouse abdominal MRI. Fasting and administration of hyoscine butylbromide (BUSC) have been proposed for bowel motion reduction, but with inconsistent results and limited efficacy assessments. Here, we evaluate these regimes for mouse abdominal MRI at high field. Methods: Thirty-two adult C57BL/6J mice were imaged on a 9.4T scanner with a FLASH sequence, acquired over 90 minutes with ~19s temporal resolution. During MRI acquisition, eight mice were injected with a low-dose and eight mice with a high-dose bolus of BUSC (0.5 and 5 mg/kg, respectively). Eight mice were food deprived for 4.5-6.5h before MRI and another group of 8 mice was injected with saline during MRI acquisition. Two expert readers reviewed the images and classified bowel motion, and quantitative voxel-wise analyses were performed for identification of moving regions. After defining the most effective protocol, high-resolution T2-weighted and diffusion-weighted images were acquired from four mice. Results: High-dose BUSC was the most effective protocol for bowel motion reduction, for up to 45 minutes. Fasting and saline protocols were not effective in suppressing bowel motion. High-resolution abdominal MRI clearly demonstrated improved image quality and ADC quantification with the high-dose BUSC protocol. Conclusion: Our data show that BUSC administration is advantageous for abdominal MRI in the mouse. Specifically, it endows significant bowel motion reduction, with relatively short onset timings after injection (~8.5 minutes) and relatively long duration of the effect (~45 minutes). These features improve the quality of high-resolution images of the mouse abdomen.

physics.med-ph↗

arXiv2020

Ultrafast functional magnetic resonance imaging reveals neuroplasticity-driven timing modulations

Functional Magnetic Resonance Imaging (fMRI) is predominantly harnessed for spatially mapping activation foci along distributed pathways. However, resolving dynamic information on activation sequence remains elusive. Here, we show an ultra-fast fMRI (ufMRI) approach - a facilitating non-invasive methodology for mapping Blood-Oxygenation-Level-Dependent (BOLD) response timings in distributed pathways with high spatiotemporal sensitivity and resolution. The mouse visual pathway was investigated under both normal and dark reared conditions. Results show that BOLD responses of normal reared mice preserve the neural input order from onset to peak times. However, modulatory effects in cortical responses due to dark rearing are only measurable at early response timings while a general delay in responses is measured at later timings. Our findings highlight the importance of robustly measuring early BOLD timings and pave the way for a better understanding and interpretation of functional MRI in healthy and aberrant conditions.

physics.med-ph↗

arXiv2020

Functional Magnetic Resonance Spectroscopy in the mouse

Functional magnetic resonance spectroscopy (fMRS) quantifies metabolic variations upon presentation of a stimulus and can therefore provide complementary information compared to functional magnetic resonance imaging (fMRI). However, to our knowledge, fMRS has not yet been performed in the mouse, despite that murine models are crucial for basic and applied research. Here, we performed fMRS experiments in the mouse, for the first time, and show the feasibility of such an approach for reliably quantifying metabolic variations. In particular, we observed metabolic variations in the superior colliculus of mice upon visual stimulation in a block paradigm commonly used for fMRI (short periods of stimulus), followed by a recovery period. We notably report a robust modulation of glutamate, as well as a modulation of NAAG, PCr and Cr. A control experiment with no stimulation reveals potential metabolic signal "drifts" that are not correlated with the functional activity, which should be taken into account when analyzing fMRS data in general. Our findings are promising for future applications of fMRS in the mouse.

physics.med-ph↗