arXiv subjects

Giovanni Volpe

Publications and source records attributed to Giovanni Volpe.

118 records · Page 7Linked to original sources

arXiv2011

Reply to Comment on "Influence of Noise on Force Measurements" [1012.5371]

Having a priori knowledge of the force acting on a noisy system it is possible to solve the issue relative to the interpretation of multiplicative noise terms (aka Ito-Stratonovich dilemma). We experimentally show that for a Brownian particle the anti-Ito convention is correct. This permits us to reconcile force measurements based on the equilibrium distribution and on the drift velocity of a Brownian particle in a diffusion gradient.

cond-mat.soft↗

arXiv2011

Microswimmers in Patterned Environments

We demonstrate with experiments and simulations how microscopic self-propelled particles navigate through environments presenting complex spatial features, which mimic the conditions inside cells, living organisms and future lab-on-a-chip devices. In particular, we show that, in the presence of periodic obstacles, microswimmers can steer even perpendicularly to an applied force. Since such behaviour is very sensitive to the details of their specific swimming style, it can be employed to develop advanced sorting, classification and dialysis techniques.

physics.bio-ph↗

arXiv2010

Force measurement in the presence of Brownian noise: Equilibrium distribution method vs. Drift method

The study of microsystems and the development of nanotechnologies require new techniques to measure piconewton and femtonewton forces at microscopic and nanoscopic scales. Amongst the challenges, there is the need to deal with the ineluctable thermal noise, which, in the typical experimental situation of a spatial diffusion gradient, causes a spurious drift. This leads to a correction term when forces are estimated from drift measurements [Phys. Rev. Lett. 104, 170602 (2010)]. Here, we provide a systematic study of such effect comparing the forces acting on various Brownian particles derived from equilibrium distribution and drift measurements. We discuss the physical origin of the correction term, its dependence on wall distance, particle radius, and its relation to the convention used to solve the respective stochastic integrals. Such correction term becomes more significant for smaller particles and is predicted to be in the order of several piconewtons for particles the size of a biomolecule.

cond-mat.stat-mech↗

arXiv2010

Influence of rotational force fields on the determination of the work done on a driven Brownian particle

For a Brownian system the evolution of thermodynamic quantities is a stochastic process. In particular, the work performed on a driven colloidal particle held in an optical trap changes for each realization of the experimental manipulation, even though the manipulation protocol remains unchanged. Nevertheless, the work distribution is governed by established laws. Here, we show how the measurement of the work distribution is influenced by the presence of rotational, i.e. nonconservative, radiation forces. Experiments on particles of different materials show that the rotational radiation forces, and therefore their effect on the work distributions, increase with the particle refractive index.

cond-mat.stat-mech↗

arXiv2010

Influence of Noise on Force Measurements

We demonstrate how the ineluctable presence of thermal noise alters the measurement of forces acting on microscopic and nanoscopic objects. We quantify this effect exemplarily for a Brownian particle near a wall subjected to gravitational and electrostatic forces. Our results demonstrate that the force measurement process is prone to artifacts if the noise is not correctly taken into account.

cond-mat.soft↗

arXiv2009

Novel perspectives for the application of total internal reflection microscopy

Total Internal Reflection Microscopy (TIRM) is a sensitive non-invasive technique to measure the interaction potentials between a colloidal particle and a wall with femtonewton resolution. The equilibrium distribution of the particle-wall separation distance z is sampled monitoring the intensity I scattered by the Brownian particle under evanescent illumination. Central to the data analysis is the knowledge of the relation between I and the corresponding z, which typically must be known a priori. This poses considerable constraints to the experimental conditions where TIRM can be applied (short penetration depth of the evanescent wave, transparent surfaces). Here, we introduce a method to experimentally determine I(z) by relying only on the distance-dependent particle-wall hydrodynamic interactions. We demonstrate that this method largely extends the range of conditions accessible with TIRM, and even allows measurements on highly reflecting gold surfaces where multiple reflections lead to a complex I(z).

physics.optics↗

arXiv2009

Quantitative assessment of non-conservative radiation forces in an optical trap

The forces acting on an optically trapped particle are usually assumed to be conservative. However, the presence of a non-conservative component has recently been demonstrated. Here we propose a technique that permits one to quantify the contribution of such non-conservative component. This is an extension of a standard optical tweezers calibration technique and, therefore, can easily become a standard test to verify the conservative optical force assumption. Using this technique we have analyzed different-sized optically trapped particles under different trapping conditions. We conclude that the non-conservative effects are effectively negligible and do not affect the standard calibration procedure, unless for extremely low-power trapping, far away from the trapping regimes usually used in experiments.

cond-mat.stat-mech↗

arXiv2008

Thermal noise suppression: how much does it cost?

In order to stabilize the behavior of noisy systems, confining it around a desirable state, an effort is required to suppress the intrinsic noise. This noise suppression task entails a cost. For the important case of thermal noise in an overdamped system, we show that the minimum cost is achieved when the system control parameters are held constant: any additional deterministic or random modulation produces an increase of the cost. We discuss the implications of this phenomenon for those overdamped systems whose control parameters are intrinsically noisy, presenting a case study based on the example of a Brownian particle optically trapped in an oscillating potential.

cond-mat.stat-mech↗

arXiv2007

Brownian motion in a non-homogeneous force field and photonic force microscope

The Photonic Force Microscope (PFM) is an opto-mechanical technique based on an optical trap that can be assumed to probe forces in microscopic systems. This technique has been used to measure forces in the range of pico- and femto-Newton, assessing the mechanical properties of biomolecules as well as of other microscopic systems. For a correct use of the PFM, the force field to measure has to be invariable (homogeneous) on the scale of the Brownian motion of the trapped probe. This condition implicates that the force field must be conservative, excluding the possibility of a rotational component. However, there are cases where these assumptions are not fulfilled Here, we show how to improve the PFM technique in order to be able to deal with these cases. We introduce the theory of this enhanced PFM and we propose a concrete analysis workflow to reconstruct the force field from the experimental time-series of the probe position. Furthermore, we experimentally verify some particularly important cases, namely the case of a conservative or rotational force-field.

physics.data-an↗

arXiv2007

Singular point characterization in microscopic flows

We suggest an approach to microrheology based on optical traps in order to measure fluid fluxes around singular points of fluid flows. We experimentally demonstrate this technique, applying it to the characterization of controlled flows produced by a set of birefringent spheres spinning due to the transfer of light angular momentum. Unlike the previous techniques, this method is able to distinguish between a singular point in a complex flow and the absence of flow at all; furthermore it permits us to characterize the stability of the singular point.

physics.optics↗