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Gregor Knoener

Publications and source records attributed to Gregor Knoener.

4 recordsLinked to original sources

Picolitre viscometry using optically rotated particles

Important aspects in the field of microrheology are the studies of the viscosity of fluids within structures with micron dimensions and fluid samples where only microlitre volumes are available. We have quantitatively investigated the performance and accuracy of a microviscometer based on rotating optical tweezers, that requires as little as one microlitre of sample. We have characterised our microviscometer, including effects due to heating, and demonstrated its ability to perform measurements over a large dynamic range of viscosities (at least two orders of magnitude). We have also inserted a probe particle through the membrane of a cell and measured the viscosity of the intramembranous contents. Viscosity measurements of tears have also been made with our microviscometer, which demonstrate its potential use to study un-stimulated eye fluid.

physics.optics

Refractometry of organosilica microspheres

The refractive index of novel organosilica (nano/micro)material is determined using two methods. The first method is based on analysis of optical extinction efficiency of organosilica beads versus wavelength, which is obtained by a standard laboratory spectrometer. The second method relies on the measurable trapping potential of these beads in the focused light beam (laser tweezers). Polystyrene beads were used to test these methods, and the determined dispersion curves of refractive index values have been found accurate. The refractive index of organosilica beads has been determined to range from 1.60-1.51 over the wavelength range of 300-1100 nm.

physics.optics

Measurement of refractive index of single microparticles

The refractive index of single microparticles is derived from precise measurement and rigorous modeling of the stiffness of a laser trap. We demonstrate the method for particles of four different materials with diameters from 1.6 to 5.2 microns and achieve an accuracy of better than 1%. The method greatly contributes as a new characterization technique because it works best under conditions (small particle size, polydispersion) where other methods, such as absorption spectroscopy, start to fail. Particles need not be transferred to a particular fluid, which prevents particle degradation or alteration common in index matching techniques. Our results also show that advanced modeling of laser traps accurately reproduces experimental reality.

physics.optics

Measurement of the total optical angular momentum transfer in optical tweezers

We describe a way to determine the total angular momentum, both spin and orbital, transferred to a particle trapped in optical tweezers. As an example an LG02 mode of a laser beam with varying degrees of circular polarisation is used to trap and rotate an elongated particle with a well defined geometry. The method successfully estimates the total optical torque applied to the particle. For this technique, there is no need to measure the viscous drag on the particle, as it is an optical measurement. Therefore, knowledge of the particle's size and shape, as well as the fluid's viscosity, is not required.

physics.optics