Hancheng Shao | Searcharxiv

arXiv2024

Asgard/NOTT: L-band nulling interferometry at the VLTI. II. Warm optical design and injection system

Asgard/NOTT (previously Hi-5) is a European Research Council (ERC)-funded project hosted at KU Leuven and a new visitor instrument for the Very Large Telescope Interferometer (VLTI). Its primary goal is to image the snow line region around young stars using nulling interferometry in the L-band (3.5 to 4.0)$μ$m, where the contrast between exoplanets and their host stars is advantageous. The breakthrough is the use of a photonic beam combiner, which only recently allowed the required theoretical raw contrast of $10^{-3}$ in this spectral range. Nulling interferometry observations of exoplanets also require a high degree of balancing between the four pupils of the VLTI in terms of intensity, phase, and polarization. The injection into the beam combiner and the requirements of nulling interferometry are driving the design of the warm optics and the injection system. The optical design up to the beam combiner is presented. It offers a technical solution to efficiently couple the light from the VLTI into the beam combiner. During the coupling, the objective is to limit throughput losses to 5% of the best expected efficiency for the injection. To achieve this, a list of different loss sources is considered with their respective impact on the injection efficiency. Solutions are also proposed to meet the requirements on beam balancing for intensity, phase, and polarization. The different properties of the design are listed, including the optics used, their alignment and tolerances, and their impact on the instrumental performances in terms of throughput and null depth. The performance evaluation gives an expected throughput loss of less than <6.4% of the best efficiency for the injection and a null depth of $\sim2.10^{-3}$, mainly from optical path delay errors outside the scope of this work.

astro-ph.IM↗

arXiv2023

High Accuracy Protein Identification: Fusion of solid-state nanopore sensing and machine learning

Proteins are arguably the most important class of biomarkers for health diagnostic purposes. Label-free solid-state nanopore sensing is a versatile technique for sensing and analysing biomolecules such as proteins at single-molecule level. While molecular-level information on size, shape, and charge of proteins can be assessed by nanopores, the identification of proteins with comparable sizes remains a challenge. Here, we present methods that combine solid-state nanopore sensing with machine learning to address this challenge. We assess the translocations of four similarly sized proteins using amplifiers with bandwidths (BWs) of 100 kHz (sampling rate=200 ksps) and 10 MHz (sampling rate=40 Msps), the highest bandwidth reported for protein sensing, using nanopores fabricated in <10 nm thick silicon nitride membranes. F-values of up to 65.9% and 83.2% (without clustering of the protein signals) were achieved with 100 kHz and 10 MHz BW instruments, respectively, for identification of the four proteins. The accuracy of protein identification was significantly improved by grouping the signals into several clusters depending on the event features, resulting in F-value and specificity reaching as high as 88.7% and 96.4%, respectively, for combinations of four proteins. The combined improvement in sensor signals through the use of high bandwidth instruments, advanced clustering, machine learning, and other advanced data analysis methods allows identification of proteins with high accuracy.

physics.bio-ph↗