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Hiroharu Ajiro

Publications and source records attributed to Hiroharu Ajiro.

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Robotic System for Chemical Experiment Automation with Dual Demonstration of End-effector and Jig Operations

While robotic automation has demonstrated remarkable performance, such as executing hundreds of experiments continuously over several days, designing synchronized motions between the robot and experimental jigs remains challenging, especially for flexible experimental automation. This challenge stems from the fact that even minor changes in experimental conditions often require extensive reprogramming of both robot motions and jig control commands. Previous systems lack the flexibility to accommodate frequent updates, limiting their practical utility in actual laboratories. To update robotic automation systems flexibly by chemists, we propose a concept that enables the automation of experiments by utilizing dual demonstrations of robot motions and jig operations by chemists. To verify this concept, we developed a chemical-experiment-automation system consisting of jigs to assist the robot in experiments, a motion-demonstration interface, a jig-control interface, and a mobile manipulator. We validate the concept through polymer-synthesis experiments, focusing on critical liquid-handling tasks such as pipetting and dilution. The experimental results indicate high reproducibility of the demonstrated motions and robust task-success rates. This comprehensive concept not only simplifies the robot programming process for chemists but also provides a flexible and efficient solution to accommodate a wide range of experimental conditions, providing a practical framework for intuitive and adaptable robotic laboratory automation. Our project page is available at: https://sasakihikaru.github.io/Chemical-Experiment-Automation-with-Dual-Demonstration/.

cs.RO

Tuneable drug-loading capability of chitosan hydrogels with varied network architectures

Advanced bioactive systems with defined macroscopic properties and spatio-temporal sequestration of extracellular biomacromolecules are highly desirable for next generation therapeutics. Here, chitosan hydrogels were prepared with neutral or negatively-charged crosslinkers in order to promote selective electrostatic complexation with charged drugs. Chitosan (CT) was functionalised with varied dicarboxylic acids, such as tartaric acid (TA), poly(ethylene glycol) bis(carboxymethyl) ether (PEG), 1.4-Phenylenediacetic acid (4Ph) and 5-Sulfoisophthalic acid monosodium salt (PhS), whereby PhS was hypothesised to act as a simple mimetic of heparin. ATR FT-IR showed the presence of C=O amide I, N-H amide II and C=O ester bands, providing evidence of covalent network formation. The crosslinker content was reversely quantified by 1H-NMR on partially-degraded network oligomers, so that 18 mol% PhS was exemplarily determined. Swellability, compressability, material morphology, and drug-loading capability were successfully adjusted based on the selected network architecture. Here, hydrogel incubation with model drugs of varied electrostatic charge, i.e. allura red (AR, --), methyl orange (MO, -) or methylene blue (MB, +), resulted in direct hydrogel-dye electrostatic complexation. Importantly, the cationic compound, MB, showed different incorporation behaviours, depending on the electrostatic character of the selected crosslinker. In light of this tuneable drug-loading capability, these CT hydrogels would be highly attractive as drug reservoirs towards e.g. the fabrication of tissue models in vitro.

physics.chem-ph