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James N. Caron

Publications and source records attributed to James N. Caron.

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Modifications to Image Phase Alignment Super-sampling Produce up to 4.4 times Increased Image Resolution

Image Phase Alignment Super-sampling (ImPASS) is a computational method for combining displaced low-resolution images into a single high-resolution image. The general steps include measuring the relative displacements, up-sampling, aligning and combining the images, followed by a blind deconvolution. Previous ImPASS studies have shown that the resulting image resolution can significantly subceed the diffraction limit of the imaging system. Characteristics that potentially limit the processed image resolution include optical parameters, detector noise, image alignment accuracy, or deconvolution parameters. In this report, modifications have been made to the algorithm to improve the image alignment accuracy and deconvolution. Applications of the modified algorithm improved image resolution by a factor up to 1.81. Compared to the original image resolution, the modified ImPASS achieved a resolution improvement factor up to 4.41 while subceeding the diffraction limit by a factor of 2.57. This suggests that limitations imposed by the physical properties of the system have not yet been reached, and further improvement of the algorithm is warranted.

physics.optics

Application of Super-Sampling to Microscopy Images Produces Image Resolution below Optical Diffraction Limit

Image Phase Alignment Super-Sampling (ImPASS) is a computational imaging algorithm for converting a sequence of displaced low-resolution images into a single high-resolution image. The method consists of a unique combination of Phase Correlation image registration and SeDDaRA blind deconvolution. The method has previously been validated in simulations and applied successfully to images captured in a laboratory setting. As discussed here, the performance of ImPASS surpasses similar methods that provide quantitative results. ImPASS is applied for the first time to images taken by a widefield microscope, requiring no customization other than a translation stage, to determine if this approach can subceed the diffraction limit for this application. The 80-frame image sets had as targets a slide with a slice of Porcine Cornea, and a standard US Air Force resolution chart, providing quantitative and quantitative assessments. The sets were up-sampled by a factor of eight, aligned, combined, and processed. The measurement revealed that image resolution improved by a factor of 2.68 and subceeded the diffraction limit by a factor of 1.79.

physics.optics