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Jared Houghtaling

Publications and source records attributed to Jared Houghtaling.

2 recordsLinked to original sources

Toward AI-Ready Medical Imaging Data

Medical imaging data plays a vital role in disease diagnosis, monitoring, and clinical research discovery. Biomedical data managers and clinical researchers must navigate a complex landscape of medical imaging infrastructure, input/output tools and data reliability workflow configurations taking months to operationalize. While standard formats exist for medical imaging data, standard operating procedures (SOPs) for data management are lacking. These data management SOPs are key for developing Findable, Accessible, Interoperable, and Reusable (FAIR) data, a prerequisite for AI-ready datasets. The National Institutes of Health (NIH) Bridge to Artificial Intelligence (Bridge2AI) Standards Working Group members and domain-expert stakeholders from the Bridge2AI Grand Challenges teams developed data management SOPs for the Digital Imaging and Communications in Medicine (DICOM) format. We describe novel SOPs applying to both static and cutting edge video imaging modalities. We emphasize steps required for centralized data aggregation, validation, and de-identification, including a review of new defacing methods for facial DICOM scans, anticipating adversarial AI/ML data re-identification methods. Data management vignettes based on Bridge2AI datasets include example parameters for efficient capture of a wide modality spectrum, including datasets from new ophthalmology retinal scans DICOM modalities.

q-bio.OT

Real-time shape approximation and 5-D fingerprinting of single proteins

This work exploits the zeptoliter sensing volume of electrolyte-filled nanopores to determine, simultaneously and in real time, the approximate shape, volume, charge, rotational diffusion coefficient, and dipole moment of individual proteins. We have developed the theory for a quantitative understanding and analysis of modulations in ionic current that arise from rotational dynamics of single proteins as they move through the electric field inside a nanopore. The resulting multi-parametric information raises the possibility to characterize, identify, and quantify individual proteins and protein complexes in a mixture. This approach interrogates single proteins in solution and determines parameters such as the approximate shape and dipole moment, which are excellent protein descriptors and cannot be obtained otherwise from single protein molecules in solution. Taken together, this five-dimensional characterization of biomolecules at the single particle level has the potential for instantaneous protein identification, quantification, and possibly sorting with implications for structural biology, proteomics, biomarker detection, and routine protein analysis.

q-bio.BM