arXiv subjects

Jianan Huang

Publications and source records attributed to Jianan Huang.

8 recordsLinked to original sources

arXiv2026

Automatic control of gold nanoparticle clustering in plasmonic nanopores based on Raman spectroscopic feedback

Plasmonic nanopores have been used to trap single nanoparticles using electric bias for single-molecule surface enhanced Raman spectroscopic (SM-SERS) detection and sequencing. While they exhibit high sensitivity, removal of nanoparticle clusters in the nanopore is so labor intensive that it hampers biosensing applications. Here, our approach is to use a microcontroller to change the electric voltage to remove the nanoparticle clusters in the nanopore with feedback by the Raman spectra. The SM-SERS spectra of the molecules adsorbed on nanoparticle surface can be acquired over a sufficiently long period, so that the microcontroller can analyze them with <100 ms and trigger the voltage change to remove the nanoparticle cluster. Our system is promising for automatic and high-throughput SM-SERS detection and analysis of DNA and proteins.

physics.optics↗

arXiv2026

SERS study of single-live-cell electrical permeabilization dynamics via plasmonic nanotubes

There is a growing demand for minimally invasive methods to analyze intracellular processes and signaling activities in individual living cells, including the identification of tumorigenic cell subpopulations. However, most conventional analytical methods require cell lysis, precluding repeated measurements in the same cell over time, or rely on exogenous labels and reporters that may perturb cellular function. Various applications based on vertical nanotubes have been developed that enable live cell monitoring and analysis by electroporation with low voltages. However, the extent and duration of membrane permeability and kinetics of membrane repair remain elusive. Here, we built a plasmonic platform with the capacity of surface enhanced Raman spectroscopy (SERS) to monitor the electroporation-induced membrane permeability dynamics in individual live cells attached onto 100-nm diameter nanotubes of 2 um height. Fibronectin was employed as extracellular matrix (ECM)-coating to facilitate cell attachment onto nanotubes. Using fluorescent-dye delivery as an independent validation method, we show that the fabricated nanostructures induce localized electrical permeabilization of the plasma membrane and enable monitoring of its subsequent recovery. We further use SERS to track molecular changes at the membrane during permeabilization and resealing. The SERS spectra provide molecular-level insight into changes in membrane-associated components and the ECM during electroporation and subsequent membrane recovery. Real time monitoring of pulse induced molecular changes holds great promise for characterizing intracellular signaling, cellular states, and cellular heterogeneity at the single cell level, including the identification of tumorigenic subpopulations. This capacity could facilitate the development of novel biosensing assay.

physics.app-ph↗

arXiv2026

Label-free SERS Discrimination of Native Proline Hydroxylation at Single-molecule peptide by Deep Learning-assisted plasmonic nanopore

Post-translational modifications (PTMs) play essential roles in regulating protein structure, function, and cellular signalling. However, peptide level discrimination of hydroxylation at the single-molecule level remains difficult. Here, we report a particle-in-pore single-molecule surface-enhanced Raman spectroscopy (SERS) platform combined with peak occurrence frequency (POF) analysis and a one-dimensional convolutional neural network (1D-CNN) for discriminating hydroxylated and non-hydroxylated HIF peptide fragments. Three peptide pairs containing the Pro-564 hydroxylation site, with lengths of 7, 9, and 15 amino acids (AAs), were investigated. POF analysis revealed reproducible hydroxylation-dependent spectral changes in the 7AA and 9AA peptide pairs, which were attributed to changes in adsorption conformation and surface interactions. CNN-based classification achieved post-evaluation accuracies of 72.98%, 78.55%, and 89.74% for the 7AA, 9AA, and 15AA peptide pairs, respectively, with AUC values above 0.80 for all the pairs, indicating a reliable discrimination. Gradient-weighted feature visualization further showed that CNN-sensitive regions overlapped with recurrent POF features, supporting the chemical relevance of the learned classification patterns. Notably, for the 15AA peptide pair, the enhanced citrate-associated band suggests that hydroxylation can substantially alter peptide-gold nanoparticle adsorption behaviour. This adsorption-mediated effect may amplify hydroxylation-induced spectral differences and contribute to the improved discrimination accuracy despite the increased structural complexity. These results demonstrate that the particle-in-pore sensor, assisted by deep learning, can capture hydroxylation-induced spectral and adsorption changes in peptide fragments, providing a promising strategy for ultrasensitive analysis of weak PTM signatures in peptides.

physics.bio-ph↗

arXiv2026

Improving Generalization on Cybersecurity Tasks with Multi-Modal Contrastive Learning

The use of ML in cybersecurity has long been impaired by generalization issues: Models that work well in controlled scenarios fail to maintain performance in production. The root cause often lies in ML algorithms learning superficial patterns (shortcuts) rather than underlying cybersecurity concepts. We investigate contrastive multi-modal learning as a first step towards improving ML performance in cybersecurity tasks. We aim at transferring knowledge from data-rich modalities, such as text, to data-scarce modalities, such as payloads. We set up a case study on threat classification and propose a two-stage multi-modal contrastive learning framework that uses textual vulnerability descriptions to guide payload classification. First, we construct a semantically meaningful embedding space using contrastive learning on descriptions. Then, we align payloads to this space, transferring knowledge from text to payloads. We evaluate the approach on a large-scale private dataset and a synthetic benchmark built from public CVE descriptions and LLM-generated payloads. The methodology appears to reduce shortcut learning over baselines on both benchmarks. We release our synthetic benchmark and source code as open source.

cs.CR↗

arXiv2025

Bridging Optical Sensing and Wearable Health Monitoring: A Functionalized Plasmonic Nanopillar for Non-Invasive Sweat Glucose Detection

Continuous glucose monitoring (CGM) is vital for diabetes care, but current systems rely on invasive implants or electrochemical sensors that often cause discomfort and skin irritation. Non-invasive alternatives remain limited by low sensitivity and poor compatibility with complex sweat environments, highlighting the urgent need for a comfortable and reliable solution. Here, we report the development of a wearable optical sensor watch that integrates surface plasmon resonance (SPR) technology with a functionalized silver-coated silicon nanowire (Ag/SiNW) substrate for real-time, non-invasive glucose monitoring in sweat. The nanostructured sensor is functionalized with 4-mercaptophenylboronic acid (4-MPBA), enabling selective glucose capture and optical signal transduction through both Raman scattering and SPR shift. The dual-mode detection strategy was systematically optimized, and a miniaturized SPR system operating at 638 nm was successfully integrated into a wearable watch format with wireless data transmission to a mobile application. This wearable device demonstrated excellent sensitivity (LOD down to 0.12 mM) and high selectivity in detecting glucose within physiological sweat concentration ranges. Human subject trials confirmed its applicability in real-life scenarios. This study offers a promising non-invasive alternative to traditional CGM and highlights the potential of integrating nanophotonic sensors with wearable platforms for continuous health monitoring and personalized medicine.

physics.optics↗

arXiv2025

IoT-AMLHP: Aligned Multimodal Learning of Header-Payload Representations for Resource-Efficient Malicious IoT Traffic Classification

Traffic classification is crucial for securing Internet of Things (IoT) networks. Deep learning-based methods can autonomously extract latent patterns from massive network traffic, demonstrating significant potential for IoT traffic classification tasks. However, the limited computational and spatial resources of IoT devices pose challenges for deploying more complex deep learning models. Existing methods rely heavily on either flow-level features or raw packet byte features. Flow-level features often require inspecting entire or most of the traffic flow, leading to excessive resource consumption, while raw packet byte features fail to distinguish between headers and payloads, overlooking semantic differences and introducing noise from feature misalignment. Therefore, this paper proposes IoT-AMLHP, an aligned multimodal learning framework for resource-efficient malicious IoT traffic classification. Firstly, the framework constructs a packet-wise header-payload representation by parsing packet headers and payload bytes, resulting in an aligned and standardized multimodal traffic representation that enhances the characterization of heterogeneous IoT traffic. Subsequently, the traffic representation is fed into a resource-efficient neural network comprising a multimodal feature extraction module and a multimodal fusion module. The extraction module employs efficient depthwise separable convolutions to capture multi-scale features from different modalities while maintaining a lightweight architecture. The fusion module adaptively captures complementary features from different modalities and effectively fuses multimodal features.

cs.NI↗

arXiv2024

Label-free SERS Discrimination of Proline from Hydroxylated Proline at Single-molecule Level Assisted by a Deep Learning Model

Discriminating the low-abundance hydroxylated proline from hydroxylated proline is crucial for monitoring diseases and eval-uating therapeutic outcomes that require single-molecule sensors. While the plasmonic nanopore sensor can detect the hydrox-ylation with single-molecule sensitivity by surface enhanced Raman spectroscopy (SERS), it suffers from intrinsic fluctuations of single-molecule signals as well as strong interference from citrates. Here, we used the occurrence frequency histogram of the single-molecule SERS peaks to extract overall dataset spectral features, overcome the signal fluctuations and investigate the citrate-replaced plasmonic nanopore sensors for clean and distinguishable signals of proline and hydroxylated proline. By ligand exchange of the citrates by analyte molecules, the representative peaks of citrates decreased with incubation time, prov-ing occupation of the plasmonic hot spot by the analytes. As a result, the discrimination of the single-molecule SERS signals of proline and hydroxylated proline was possible with the convolutional neural network model with 96.6% accuracy.

physics.chem-ph↗

arXiv2024

Deep learning assisted SERS detection of prolines and hydroxylated prolines using nitrilotriacetic acid functionalized gold nanopillars

Proline (Pro) is one kind of proteinogenic amino acid and an important signaling molecule in the process of metabolism. Hydroxyproline (Hyp) is a product on Pro oxygen sensing post-translational modification (PTM), which is efficiently modulated tumor cells for angiogenesis. Distinguishing between Pro and Hyp is crucial for diagnosing connective tissue disorders, as elevated levels of Hyp can indicate abnormal collagen metabolism, often associated with diseases like osteogenesis imperfecta or fibrosis. However, there is a very small difference between molecular structures of Pro and Hyp, which is a big challenge for current detection technologies to distinguish them. For surface-enhanced Raman scattering (SERS) sensors, the similar molecule structure leads to similar Raman spectra that are difficult to distinguish. Furthermore, another problem is the weak affinity between amino acids sample and SERS-active substrates by physical adsorption. The selecting capturing of Pro and Hyp in the mixture of amino acids is not easy to achieve. In this work, we designed a new method for Pro and Hyp specifical detection and recognition by using gold nanopillars as the SERS substrate and combing nitrilotriacetic acid (NTA) with nickel (Ni) to form NTA-Ni structure as a specifical affinity agent. One side of NTA-Ni was attached to gold nanopillars through thiol binding. Another side captured the amino acids using reversible binding by receptor-ligand interaction between Ni and amino acids. Because of the different binding time with NTA-Ni and amino acids, the sensor can recognize Pro and Hyp from amino acids mixture. Then we used automatic peak assignment program for data analysis and machine learning model to distinguish between Pro and Hyp. The label-free SERS detection of amino acids PTM using gold nanopillars provides a potential method to further biomolecule detection and specifical capture.

q-bio.BM↗