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Kedar Khare

Publications and source records attributed to Kedar Khare.

17 recordsLinked to original sources

Terahertz Fourier Ptychographic Imaging

High-resolution imaging in the terahertz (THz) spectral range remains fundamentally constrained by the limited numerical apertures of currently existing state-of-the-art imagers, which restricts its applicability across many fields, such as imaging in complex media or nondestructive testing. To address this challenge, we introduce a proof-of-concept implementation of THz Fourier Ptychographic imaging to enhance spatial resolution without requiring extensive hardware modifications. Our method employs a motorized kinematic mirror to generate a sequence of controlled, multi-angle plane-wave illuminations, with each resulting oblique-illumination intensity image encoding a limited portion of the spatial-frequency content of the target imaging sample. These measurements are combined in the Fourier domain using an aberration-corrected iterative phase-retrieval algorithm integrated with an efficient illumination calibration scheme, which enables the reconstruction of resolution-enhanced amplitude and phase images through the synthetic expansion of the effective numerical aperture. Our work establishes a robust framework for high-resolution THz imaging and paves the way for a wide array of applications in materials characterization, spectroscopy, and non-destructive evaluation.

physics.optics

Learning phase diversity for solving ill-posed inverse problems in imaging

Inverse problems in imaging are typically ill-posed and are usually solved by employing regularized optimization techniques. The usage of appropriate constraints can restrict the solution space, thus making it feasible for a reconstruction algorithm to find a meaningful solution. In recent years, deep network based ideas aimed at learning the end-to-end mapping between the raw measurements and the target image have gained popularity. In the learning approach, the functional relationship between the measured raw data and the solution image are learned by training a deep network with prior examples. While this approach allows one to significantly increase the real-time operational speed, it does not change the nature of the underlying ill-posed inverse problem. It is well-known that availability of diverse non-redundant data via additional measurements can generically improve the robustness of the reconstruction algorithms. The multiple data measurements, however, typically demand additional hardware and complex system setups that are not desirable. In this work, we note that in both incoherent and coherent optical imaging, the irradiance patterns corresponding to two phase diverse measurements associated with the same test object have implicit local correlation which may be learned. A physics informed data augmentation scheme is then described where a trained network is used for generating a phase diverse pseudo-data based on a ground truth data frame. The true data along with the augmented pesudo-data are observed to provide high quality inverse solutions with simpler reconstruction algorithms. We validate this approach for both incoherent and coherent optical imaging (or phase retrieval) configurations with vortex phase as a diversity mechanism. Our results may open new avenues for leaner high-fidelity computational imaging systems across a broad range of applications.

eess.IV

Super-resolution imaging of azimuthal features with illumination carrying OAM

Super-resolution imaging refers to imaging techniques that surpass the Rayleigh resolution limit. One standard way to achieve super-resolution is by structuring the phase of the field illuminating the object. Although super-resolution techniques are already employed in commercial imaging devices, intense research efforts continue to enhance the resolution even further. In this work, we show that if the field illuminating the object is structured in the azimuthal coordinate--such as a field carrying orbital angular momentum (OAM)--the azimuthal features of the object can be imaged with enhanced imaging resolution. We experimentally demonstrate it with two objects, namely, an azimuthal double-slit and a Siemens star. We find that for a given azimuthal feature, there is an optimum OAM mode index of the illumination that gives the best imaging resolution. Super-resolution imaging of azimuthal feature can have important implications, especially for some biological objects that are known to have predominantly azimuthal features.

physics.app-ph

Modal complexity as a metric for Anderson localization

We present a thorough study of the complexity of optical localized modes in two-dimensional disordered photonic crystals. Direct experimental measurements of complexity were made using an interferometric setup that allowed for extraction of phases and, hence, complex-valued wavefunctions. The comparison of experimental and theoretical results allows us to propose a metric for Anderson localization based on the average value and statistical distribution of complexity. Being an alternative to other known criteria of localization, the proposed metric exploits the openness of the disordered medium and provides a quantitative characterization of the degree of localization allowing for determining the localization length.

physics.optics

3D Reconstruction of unstained cells from a single defocused hologram

We investigate the problem of 3D complex field reconstruction corresponding to unstained red blood cells (RBCs) with a single defocused off-axis digital hologram. We employ recently introduced mean gradient descent (MGD) optimization framework, to solve the 3D recovery problem. While investigating volume recovery problem for a continuous phase object like RBC, we came across an interesting feature of the back-propagated field that it does not show clear focusing effect. Therefore the sparsity enforcement within the iterative optimization framework given the single hologram data cannot effectively restrict the true object volume. For phase objects, it is known that the amplitude contrast of the back-propagated object field at the focus plane is minimum and it increases at the defocus planes. We therefore use this information available in the detector field data to device weights as a function of inverse of amplitude contrast. This weight function is employed in the iterative steps of the optimization algorithm to assist the object volume localization. The experimental illustrations of 3D volume reconstruction of the healthy as well as the malaria infected RBCs are presented. The proposed methodology is simple to implement experimentally and provides an approximate tomographic solution which is axially restricted and is consistent with the object field data.

eess.IV

Quantification of Pollen Viability in Lantana camara By Digital Holographic Microscopy

Pollen grains represent the male gametes of seed plants and their viability is critical for efficient sexual reproduction in the plant life cycle. Pollen analysis is used in diverse research thematics to address a range of botanical, ecological and geological questions. More recently it has been recognized that pollen may also be a vector for transgene escape from genetically modified crops, and the importance of pollen viability in invasion biology has also been emphasized. In this work, we analyse and report an efficient visual method for assessing the viability of pollen using digital holographic microscopy (DHM). We test this method on pollen grains of the invasive Lantana camara, a well known plant invader known to most of the tropical world. We image pollen grains and show that the quantitative phase information provided by the DHM technique can be readily related to the chromatin content of the individual cells and thereby to pollen viability. Our results offer a new technique for pollen viability assessment that does not require staining, and can be applied to a number of emerging areas in plant science.

eess.IV

Robust phase retrieval with complexity-guidance for coherent X-ray imaging

Reconstruction of a stable and reliable solution from noisy incomplete Fourier intensity data recorded in a coherent X-ray imaging (CXI) experiment is a challenging problem. The Relaxed Averaged Alternating Reflections (RAAR) algorithm that is concluded with a number of Error Reduction (ER) iterations is a popular choice. The RAAR-ER algorithm is usually employed for several hundreds of times starting with independent random guesses to obtain trial solutions that are then averaged to obtain the phase retrieval transfer function (PRTF). In this paper, we examine the phase retrieval solution obtained using the RAAR-ER methodology from perspective of the complexity parameter that was introduced by us in recent works. We observe that a single run of the RAAR-ER algorithm produces a solution with higher complexity compared to what is expected based on the complexity parameter as manifested by spurious high frequency grainy artifacts in the solution that do not seem to go away completely even after a number of trial solutions are averaged. We then describe a CG-RAAR (Complexity Guided RAAR) phase retrieval method that can effectively address this inconsistency problem and provides artifact-free solutions. The CG-RAAR methodology is first illustrated with simulated unblocked noisy Fourier intensity data and later applied to centrally-blocked noisy cyanobacterium data which is available from the CXIDB database. Our simulation and experimental results using CG-RAAR suggest two important improvements over the popular RAAR-ER algorithm. The CG-RAAR solutions after the averaging procedure is more reliable in the sense that it contains smallest features consistent with the resolution estimated by the PRTF curve. Secondly, since the single run of the CG-RAAR solution does not have grainy artifacts, the number of trial solutions needed for the averaging process is reduced.

eess.IV

Quantitative phase imaging of single particles from a cryoEM micrograph

We show that de-focused single particle images recorded using a cryo-electron microscope (cryoEM) system may be processed like a Fresnel zone in-line hologram to obtain physically meaningful quantitative phase maps associated with individual particles. In particular, a region-of-interest (ROI) of the de-focused image surrounding a particle can be numerically back-propagated, in order to determine accurate de-focus information based on the sparsity-of-gradient merit function. Further with the knowledge of de-focus information, an iterative Fresnel zone phase retrieval algorithm using image sparsity constraints can accurately estimate the quantitative phase information associated with a single particle. The proposed methodology which can correct for both de-focus and spherical aberrations is a deviation from the image processing chain currently used in single particle cryoEM reconstructions. Our illustrations as presented here suggest that the phase retrieval approach applies uniformly to de-focused image data recorded using the traditional CCD detectors as well as the newer direct electron detectors.

eess.IV

True 3D reconstruction in digital holography

We examine the nature of the 3D image as obtained by replay (or back-propagation) of the object wave from the hologram recording plane to the original object volume. While recording of a hologram involves transferring information from a 3D volume to a 2D detector plane, the replay of the hologram involves creating information in a set of 3D voxels from a much smaller number of 2D detector pixels, which at first look appears to be surprising. We point out that the hologram replay process is a Hermitian transpose (and not inverse) of the hologram formation process and therefore only provides an approximation to the original 3D object function. With the knowledge of this Hermitian transpose property, we show how one may realize true 3D image reconstruction via a regularized optimization algorithm. The numerical illustrations of this optimization approach as presented here show excellent slice-by-slice tomographic 3D reconstruction of the original object under the weak scattering approximation. In particular, the reconstructed 3D image field has near-zero numerical values at voxels where the original object did not exist. We note that 3D image reconstruction of this kind cannot be achieved by the traditional physical replay process. In this sense the proposed methodology for digital holographic image reconstruction goes beyond numerically mimicking the traditional film based holographic replay. The reconstruction approach may find potential applications in a number of digital holographic imaging systems.

eess.IV

Mean Gradient Descent: An optimization approach for single-shot interferogram analysis

Complex object wave recovery from single-shot interference pattern is an important practical problem in interferometry and digital holography. The most popular single-shot interferogram analysis method involves Fourier filtering of cross-term but this method suffers from poor resolution. For obtaining full pixel resolution, it is necessary to model the object wave recovery as an optimization problem. The optimization approach typically involves minimizing a cost function consisting of a data consistency term and one or more constraint terms. Despite its potential performance advantages, this method is not used widely due to several tedious and difficult tasks such as empirical tuning of free parameters. We introduce a new optimization approach Mean gradient descent (MGD) for single-shot interferogram analysis that is simple to implement, robust and does not require any free parameters. The MGD iteration does not try to achieve minimization of any cost function but instead aims to reach a solution point where the data consistency and the constraint terms balance each other. This is achieved by iteratively progressing the solution in the direction that bisects the descent directions associated with the error and constraint terms. Numerical illustrations are shown for recovery of a step phase object from its corresponding off-axis as well as on-axis interferograms simulated with multiple noise levels. Our results show full pixel resolution as evident from the recovery of phase step and excellent rms phase accuracy relative to the ground truth phase map. The concept of MGD as presented here can potentially find applications to wider class of optimization problems.

eess.IV

Unsupervised organization of cervical cells using high resolution digital holographic microscopy

We report results on unsupervised organization of cervical cells using microscopy of Pap-smear samples in brightfield (3-channel colour) as well as high resolution quantitative phase imaging modalities. A number of morphological parameters are measured for each of the 1450 cell nuclei (from $10$ woman subjects) imaged in this study. The Principal Component Analysis (PCA) methodology applied to this data shows that the cell image clustering performance improves significantly when brightfield as well as phase information is utilized for PCA as compared to when brightfield-only information is used. The results point to the feasibility of an image-based tool that will be able to mark suspicious cells for further examination by the pathologist. More importantly our results suggest that the information in quantitative phase images of cells that is typically not used in clinical practice is valuable for automated cell classification applications in general.

eess.IV

Phase retrieval with complexity guidance

Iterative phase retrieval methods based on the Gerchberg-Saxton (GS) or Fienup algorithm require a large number of iterations to converge to a meaningful solution. For complex-valued or phase objects, these approaches also suffer from stagnation problems where the solution does not change much from iteration to iteration but the resultant solution shows artifacts such as presence of a twin. We introduce a complexity parameter $ζ$ that can be computed directly from the Fourier magnitude data and provides a measure of fluctuations in the desired phase retrieval solution. It is observed that when initiated with a uniformly random phase map, the complexity of the Fienup solution containing stagnation artifacts stabilizes at a numerical value that is much higher than $ζ$. We propose a modified Fienup algorithm that uses a controlled sparsity enhancing step such that in every iteration the complexity of the resulting solution is explicitly made close to $ζ$. This approach which we refer to as complexity guided phase retrieval (CGPR) is seen to significantly reduce the number of phase retrieval iterations required for convergence to a meaningful solution and automatically addresses the stagnation problems. The CGPR methodology can enable new applications of iterative phase retrieval that are considered practically difficult due to large number of iterations required for a reliable phase recovery.

eess.IV

Angular Lens

We propose a single phase-only optical element that transforms different orbital angular momentum (OAM) modes into localized spots at separated angular positions on a transverse plane. We refer to this element as an angular lens since it separates out OAM modes in a manner analogous to how a converging lens separates out transverse wave-vector modes at the focal plane. We also simulate the proposed angular lens using a spatial light modulator and experimentally demonstrate its working. Our work can have important implications for OAM-based classical and quantum communication applications.

physics.optics

Optimal vector beams maintaining robust intensity profile on propagation through turbulence

We report experiments on propagation of scalar and vector optical beams through random phase screens mimicking turbulence and show that the intensity profile of the beam containing a C-point polarization singularity shows maximally robust behavior. This observation is explained in terms of the polarization and orbital angular momentum (OAM) diversity in the beam. The $l = 0$ and $l = 1$ OAM states whose vector combination leads to the C-point singularity are seen to produce complementary speckle intensity patterns with significant negative correlation on propagation through a random phase screen. This unique property of C-point singularity makes it superior to other inhomogeneous polarization states as demonstrated in our experiments. The results provide an important generic guideline for designing beams that can maintain optimally robust beam intensity profile on passing through random phase fluctuations and are expected to have a number of applications.

physics.optics

Dictionary based Approach to Edge Detection

Edge detection is a very essential part of image processing, as quality and accuracy of detection determines the success of further processing. We have developed a new self learning technique for edge detection using dictionary comprised of eigenfilters constructed using features of the input image. The dictionary based method eliminates the need of pre or post processing of the image and accounts for noise, blurriness, class of image and variation of illumination during the detection process itself. Since, this method depends on the characteristics of the image, the new technique can detect edges more accurately and capture greater detail than existing algorithms such as Sobel, Prewitt Laplacian of Gaussian, Canny method etc which use generic filters and operators. We have demonstrated its application on various classes of images such as text, face, barcodes, traffic and cell images. An application of this technique to cell counting in a microscopic image is also presented.

cs.CV

Sparsity assisted solution to the twin image problem in phase retrieval

The iterative phase retrieval problem for complex-valued objects from Fourier transform magnitude data is known to suffer from the twin image problem. In particular, when the object support is centro-symmetric, the iterative solution often stagnates such that the resultant complex image contains the features of both the desired solution and its inverted and complex-conjugated replica. The conventional approach to address the twin image problem is to modify the object support during initial iterations which can possibly lead to elimination of one of the twin images. However, at present there seems to be no deterministic procedure to make sure that the twin image will always be very weak or absent. In this work we make an important observation that the ideal solution without the twin image is typically more sparse (in some suitable transform domain) as compared to the stagnated solution containing the twin image. We further show that introducing a sparsity enhancing step in the iterative algorithm can address the twin image problem without the need to change the object support throughout the iterative process even when the object support is centro-symmetric. In a simulation study, we use binary and gray-scale pure phase objects and illustrate the effectiveness of the sparsity assisted phase recovery in the context of the twin image problem. The results have important implications for a wide range of topics in Physics where the phase retrieval problem plays a central role.

cs.CV

Multipixel sub-shot noise phase measurement with classical light interferometry

We demonstrate accurate phase measurement from low photon level interference data using a constrained optimization method that takes into account the expected redundancy in the unknown phase function. This approach is shown to have significant noise advantage over traditional methods such as balanced homodyning or phase shifting that treat individual pixels in the interference data as independent of each other. Our interference experiments comparing the optimization method with the traditional phase shifting method show that when the same photon resources are used, the optimization method provides phase recoveries with tighter error bars. In particular, RMS phase error performance of the optimization method for low photon number data (10 photons per pixel) shows $>$ 5X noise gain over the phase shifting method. In our experiments where a laser light source is used for illumination, the results imply phase measurement with accuracy better than the conventional single pixel based shot noise limit (SNL) that assumes independent phases at individual pixels. The constrained optimization approach presented here is independent of the nature of light source and may further enhance the accuracy of phase detection when a nonclassical light source is used.

quant-ph