arXiv subjects

Leonardo Puggioni

Publications and source records attributed to Leonardo Puggioni.

7 recordsLinked to original sources

arXiv2026

Shaping nematic order in bacterial films with single-cell resolution patterning

Bacterial colonies composed of elongated cells form active nematic fluids that spontaneously self-organise into ordered domains of aligned cells and exhibit self-generated chaotic flows powered by cell growth. While their dynamics have attracted significant attention, the role of initial conditions remains largely unexplored due to a lack of precise patterning methods. Here, we harness the precision of capillary assembly to pattern Bacillus subtilis endospores into arrays with controlled positions and orientations at single-cell resolution. Upon germination and growth of cell chains, we quantify the dynamics and morphologies of the resulting bacterial films. While orthogonally seeded spores lead to chaotic dynamics, seeding them with parallel orientations yields films with high nematic order across millimetres, which subsequently synchronously buckle upon further growth. Our observations are captured by numerical simulations and a model that describes the buckling dynamics starting from the mechanical properties of individual filaments. By programming local cell orientation with single-cell precision, we finally harness nematic alignment to create macroscopic bacterial films with local optical anisotropy, via structural colouration and light polarisation. Our findings demonstrate that initial conditions play a key role and offer exciting opportunities to control the spatio-temporal organization of bacterial assemblies towards addressing open biological questions and realizing living materials with tailored properties.

physics.bio-ph↗

arXiv2025

Hydrodynamic stability and pattern formation in hexatic epithelial layers

We investigate the hydrodynamic stability and the formation of patterns in a continuum model of epithelial layers, able to account for the interplay between mechanical activity, lateral adhesion and the $6-$fold orientational order originating from the hexagonal morphology of the cells. Unlike in other models of active liquid crystals, the balance between energy injection and dissipation can here involve multiple length scales, resulting in a large spectrum of dynamical behaviors. When kinetic energy is dissipated by the cells' adhesive interactions at a rate higher that at which is injected by active stresses, the quiescent state of the cellular layer is generically stable: i.e. hydrodynamically stable regardless of its size. On the other hand, as the cellular layer becomes progressively more active, this homeostatic condition is altered by a hierarchy of pattern-forming instabilities, where the system organizes in an increasingly large number of counter-flowing lanes of fixed width. In two-dimensional periodic domains, the latter organization is itself unstable to the proliferation of vortices and the dynamics of the cellular layer becomes eventually chaotic and yet different from the more common active turbulence.

cond-mat.soft↗

arXiv2025

Collective migration and topological phase transitions in confluent epithelia

Collective epithelial migration leverages on topological rearrangements of the intercellular junctions, which allow cells to intercalate without loosing confluency. In silico studies have provided a clear indication that this process could occur via a two-step phase transition, where a hierarchy of topological excitations progressively transforms an epithelial layer from a crystalline solid to an isotropic liquid, via an intermediate hexatic liquid crystal phase. Yet, the fundamental mechanism behind this process and its implications for collective cell behavior are presently unknown. In this article, we show that the onset of collective cell migration in cell-resolved models of epithelial layers takes place via an activity-driven melting transition, characterized by an exponentially-divergent correlation length across the solid/hexatic phase boundary. Using a combination of numerical simulations and Renormalization Group analysis, we show that the availability of topologically distinct rearrangements - known as T1 and T2 processes - and of a non-thermal route to melting, renders the transition significantly more versatile and tunable than in two-dimensional passive matter. Specifically, the relative frequency of T1 and T2 processes and of the "bare" stiffness of the cell layer affect the divergence of positional correlations within a well-defined spectrum of critical behaviors. Suppressing T1 processes, changes the nature of the transition by preventing collective migration in favor of a cellular analog of surface sublimation.

cond-mat.soft↗

arXiv2023

Orientational order and topological defects in a dilute solutions of rodlike polymers at low Reynolds number

The relationship between the polymer orientation and the chaotic flow, in a dilute solution of rigid rodlike polymers at low Reynolds number, is investigated by means of direct numerical simulations. It is found that the rods tend to align with the velocity field in order to minimize the friction with the solvent fluid, while regions of rotational disorder are related to strong vorticity gradients, and therefore to the chaotic flow. The "turbulent-like" behavior of the system is therefore associated with the emergence and interaction of topological defects of the mean director field, similarly to active nematic turbulence. The analysis has been carried out in both two and three spatial dimensions.

physics.flu-dyn↗

arXiv2022

Flocking turbulence of microswimmers in confined domains

We extensively study the Toner-Tu-Swift-Hohenberg model of motile active matter by means of direct numerical simulations in a two-dimensional confined domain. By exploring the space of parameters of the model we investigate the emergence of a new state of active turbulence which occurs when the aligning interactions and the self-propulsion of the swimmers are strong. This regime of flocking turbulence is characterized by a population of few strong vortices, each surrounded by an island of coherent flocking motion. The energy spectrum of flocking turbulence displays a power-law scaling with an exponent which depends weakly on the model parameters. By increasing the confinement we observe that flocking turbulence becomes unstable: after a long transient, characterized by power-law distributed transition times, the system switches to the ordered state of a single giant vortex

physics.flu-dyn↗

arXiv2021

Enhancement of drag and mixing in a dilute solution of rodlike polymers at low Reynolds numbers

We study the dynamics of a dilute solution of rigid rodlike polymers in a viscous fluid at low Reynolds number by means of numerical simulations of a simple rheological model. We show that the rotational dynamics of polymers destabilizes the laminar flow and causes the emergence of a turbulent-like chaotic flow with a wide range of active scales. This regime displays an increased flow resistance, corresponding to a reduced mean flow at fixed external forcing, as well as an increased mixing efficiency. The latter effect is quantified by measuring the decay of the variance of a scalar field transported by the flow. By comparing the results of numerical simulations of the model in two- and three-dimensions, we show that the phenomena observed are qualitatively independent on the dimensionality of the space.

physics.flu-dyn↗

arXiv2020

Conformal invariance of weakly compressible two-dimensional turbulence

We study conformal invariance of vorticity clusters in weakly compressible two-dimensional turbulence at low Mach numbers. On the basis of very high resolution direct numerical simulation we demonstrate the scaling invariance of the inverse cascade with scaling close to Kolmogorov prediction. In this range of scales, the statistics of zero-vorticity isolines are found to be compatible with those of critical percolation, thus generalizing the results obtained in incompressible Navier-Stokes turbulence.

physics.flu-dyn↗