M. A. Gradova | Searcharxiv

arXiv2018

Methods of electron microscopy of biological and abiogenic structures in artificial gas atmospheres

This paper reviews opportunities for using electron microscopy in various gas atmospheres for the analysis and morpho-physiological modification of biological structures. The approaches that allow varying the gaseous phase content, as well as temperature, humidity, and pressure, are considered. The applicability of both kinetic and dynamic approaches to the tissue and bioinorganic structure manipulations is pointed out. The possibility of simulation of the beam-induced formation and disintegration of abiogenetic molecular structures is also mentioned as a particular case of the electron beam influence and treatment of the precursor medium in an artificial atmosphere.

physics.ins-det↗

arXiv2015

"MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics

In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance microscopy, patch clamp based on scanning probe microscopy technology, quantitative subcellular secondary ion mass spectrometry or "ion microscopy", live single-cell mass spectrometry, in situ cell-by-cell imaging, single-cell video-mass spectrometry, etc. We also consider the ways to improve the informativeness of these methods and particularly emphasize the trend at the increasing of the analysis complexity. We propose here the way to improve the efficiency of the cell trapping to the capillary during MS-path-clamp, as well as to provide laser surface ionization using laser trapping and tweezing of cells with the laser beam transmitted through the capillary as a waveguide. It is also possible to combine the above system with the microcolumn separation system or capillary electrophoresis as an optional direction of further development of the complex of analytical techniques emerging from the MS variation of patch-clamp.

q-bio.SC↗