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M. Liebel

Publications and source records attributed to M. Liebel.

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Comprehensive solar eruption analyses enabled by the tools of the SOLER project

Solar eruptions comprise of a multitude of phenomena such as flares, coronal mass ejections (CMEs), large-scale coronal waves, radio bursts, and energetic particles traveling through interplanetary space. These phenomena are observed with a variety of instrumentation, including remote sensing and in-situ detectors. Obtaining a global understanding of a solar eruption often requires the analysis of various of these different datasets, including a multitude of analysis and modeling tools and a wide range of expertise. Usually, such a comprehensive analysis can only be achieved by a skilled and broad team. The Energetic Solar Eruptions: Data and Analysis Tools (SOLER) project aims at creating a comprehensive analysis platform for the study of solar eruptions that allows a single user to easily apply analysis methods addressing various counterparts of the solar event. Therefore, each partner of the project developed Python-based software, including interfaces in the form of Jupyter Notebooks, which provides application examples and concise step-to-step documentation. In this paper we introduce the comprehensive solar-eruption-analysis infrastructure developed within the SOLER project. We explain where to find the software, how to use it, and give dedicated use-case examples of how to employ selected tools in a combined manner.

physics.space-ph

Ultraviolet direct absorption microscopy for single particle protein/nucleic acid quantification

Bio-nanoparticles are pivotal to next generation nanotherapeutics, but providing single-particle biomolecular characterization remains a crucial challenge. Herein we present ultra-violet direct absorption microscopy (UV-DAM) to tackle this challenge. UV-DAM is based on a tailored illumination scheme for absorption-only imaging, combined with a custom deep UV light source. Combined, they provide biomolecular specificity with single particle sensitivity. As such, UV-DAM provides rapid, label-free, high resolution, biochemical imaging. Enabled by these capabilities, we implement the classic nucleic acid:protein absorption assay at the single virus level where we demonstrate UV-DAM's ability to distinguish empty from DNA-loaded viral capsids based on bimolecularly specific absorption fingerprints. UV-DAM presents the translation from bulk UV-visible spectrometry to single-particle assessment, a crucial advancement for nanomedicine characterization where particle loading efficiencies are often heterogenous. Beyond this, UV-DAM is applicable to a wide range of nanomaterials or investigation of biological process with high spatio-temporal resolution and intrinsic molecular contrast.

physics.optics