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Marcin Grzegorzek

Publications and source records attributed to Marcin Grzegorzek.

32 records · Page 2Linked to original sources

TOD-CNN: An Effective Convolutional Neural Network for Tiny Object Detection in Sperm Videos

The detection of tiny objects in microscopic videos is a problematic point, especially in large-scale experiments. For tiny objects (such as sperms) in microscopic videos, current detection methods face challenges in fuzzy, irregular, and precise positioning of objects. In contrast, we present a convolutional neural network for tiny object detection (TOD-CNN) with an underlying data set of high-quality sperm microscopic videos (111 videos, $>$ 278,000 annotated objects), and a graphical user interface (GUI) is designed to employ and test the proposed model effectively. TOD-CNN is highly accurate, achieving $85.60\%$ AP$_{50}$ in the task of real-time sperm detection in microscopic videos. To demonstrate the importance of sperm detection technology in sperm quality analysis, we carry out relevant sperm quality evaluation metrics and compare them with the diagnosis results from medical doctors.

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A State-of-the-art Survey of Object Detection Techniques in Microorganism Image Analysis: From Classical Methods to Deep Learning Approaches

Microorganisms play a vital role in human life. Therefore, microorganism detection is of great significance to human beings. However, the traditional manual microscopic detection methods have the disadvantages of long detection cycle, low detection accuracy in large orders, and great difficulty in detecting uncommon microorganisms. Therefore, it is meaningful to apply computer image analysis technology to the field of microorganism detection. Computer image analysis can realize high-precision and high-efficiency detection of microorganisms. In this review, first,we analyse the existing microorganism detection methods in chronological order, from traditional image processing and traditional machine learning to deep learning methods. Then, we analyze and summarize these existing methods and introduce some potential methods, including visual transformers. In the end, the future development direction and challenges of microorganism detection are discussed. In general, we have summarized 142 related technical papers from 1985 to the present. This review will help researchers have a more comprehensive understanding of the development process, research status, and future trends in the field of microorganism detection and provide a reference for researchers in other fields.

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Applications of Artificial Neural Networks in Microorganism Image Analysis: A Comprehensive Review from Conventional Multilayer Perceptron to Popular Convolutional Neural Network and Potential Visual Transformer

Microorganisms are widely distributed in the human daily living environment. They play an essential role in environmental pollution control, disease prevention and treatment, and food and drug production. The analysis of microorganisms is essential for making full use of different microorganisms. The conventional analysis methods are laborious and time-consuming. Therefore, the automatic image analysis based on artificial neural networks is introduced to optimize it. However, the automatic microorganism image analysis faces many challenges, such as the requirement of a robust algorithm caused by various application occasions, insignificant features and easy under-segmentation caused by the image characteristic, and various analysis tasks. Therefore, we conduct this review to comprehensively discuss the characteristics of microorganism image analysis based on artificial neural networks. In this review, the background and motivation are introduced first. Then, the development of artificial neural networks and representative networks are presented. After that, the papers related to microorganism image analysis based on classical and deep neural networks are reviewed from the perspectives of different tasks. In the end, the methodology analysis and potential direction are discussed.

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A State-of-the-art Survey of Artificial Neural Networks for Whole-slide Image Analysis:from Popular Convolutional Neural Networks to Potential Visual Transformers

To increase the objectivity and accuracy of pathologists' work, artificial neural network(ANN) methods have been generally needed in the segmentation, classification, and detection of histopathological WSI. In this paper, WSI analysis methods based on ANN are reviewed. Firstly, the development status of WSI and ANN methods is introduced. Secondly, we summarize the common ANN methods. Next, we discuss publicly available WSI datasets and evaluation metrics. These ANN architectures for WSI processing are divided into classical neural networks and deep neural networks(DNNs) and then analyzed. Finally, the application prospect of the analytical method in this field is discussed. The important potential method is Visual Transformers.

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A Survey of Semen Quality Evaluation in Microscopic Videos Using Computer Assisted Sperm Analysis

The Computer Assisted Sperm Analysis (CASA) plays a crucial role in male reproductive health diagnosis and Infertility treatment. With the development of the computer industry in recent years, a great of accurate algorithms are proposed. With the assistance of those novel algorithms, it is possible for CASA to achieve a faster and higher quality result. Since image processing is the technical basis of CASA, including pre-processing,feature extraction, target detection and tracking, these methods are important technical steps in dealing with CASA. The various works related to Computer Assisted Sperm Analysis methods in the last 30 years (since 1988) are comprehensively introduced and analysed in this survey. To facilitate understanding, the methods involved are analysed in the sequence of general steps in sperm analysis. In other words, the methods related to sperm detection (localization) are first analysed, and then the methods of sperm tracking are analysed. Beside this, we analyse and prospect the present situation and future of CASA. According to our work, the feasible for applying in sperm microscopic video of methods mentioned in this review is explained. Moreover, existing challenges of object detection and tracking in microscope video are potential to be solved inspired by this survey.

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A Comparative Study of Deep Learning Classification Methods on a Small Environmental Microorganism Image Dataset (EMDS-6): from Convolutional Neural Networks to Visual Transformers

In recent years, deep learning has made brilliant achievements in Environmental Microorganism (EM) image classification. However, image classification of small EM datasets has still not obtained good research results. Therefore, researchers need to spend a lot of time searching for models with good classification performance and suitable for the current equipment working environment. To provide reliable references for researchers, we conduct a series of comparison experiments on 21 deep learning models. The experiment includes direct classification, imbalanced training, and hyperparameter tuning experiments. During the experiments, we find complementarities among the 21 models, which is the basis for feature fusion related experiments. We also find that the data augmentation method of geometric deformation is difficult to improve the performance of VTs (ViT, DeiT, BotNet and T2T-ViT) series models. In terms of model performance, Xception has the best classification performance, the ViT model consumes the least time for training, and the ShuffleNet-V2 model has the least number of parameters.

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Is the aspect ratio of cells important in deep learning? A robust comparison of deep learning methods for multi-scale cytopathology cell image classification: from convolutional neural networks to visual transformers

Cervical cancer is a very common and fatal type of cancer in women. Cytopathology images are often used to screen for this cancer. Given that there is a possibility that many errors can occur during manual screening, a computer-aided diagnosis system based on deep learning has been developed. Deep learning methods require a fixed dimension of input images, but the dimensions of clinical medical images are inconsistent. The aspect ratios of the images suffer while resizing them directly. Clinically, the aspect ratios of cells inside cytopathological images provide important information for doctors to diagnose cancer. Therefore, it is difficult to resize directly. However, many existing studies have resized the images directly and have obtained highly robust classification results. To determine a reasonable interpretation, we have conducted a series of comparative experiments. First, the raw data of the SIPaKMeD dataset are pre-processed to obtain standard and scaled datasets. Then, the datasets are resized to 224 x 224 pixels. Finally, 22 deep learning models are used to classify the standard and scaled datasets. The results of the study indicate that deep learning models are robust to changes in the aspect ratio of cells in cervical cytopathological images. This conclusion is also validated via the Herlev dataset.

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GasHisSDB: A New Gastric Histopathology Image Dataset for Computer Aided Diagnosis of Gastric Cancer

Background and Objective: Gastric cancer has turned out to be the fifth most common cancer globally, and early detection of gastric cancer is essential to save lives. Histopathological examination of gastric cancer is the gold standard for the diagnosis of gastric cancer. However, computer-aided diagnostic techniques are challenging to evaluate due to the scarcity of publicly available gastric histopathology image datasets. Methods: In this paper, a noble publicly available Gastric Histopathology Sub-size Image Database (GasHisSDB) is published to identify classifiers' performance. Specifically, two types of data are included: normal and abnormal, with a total of 245,196 tissue case images. In order to prove that the methods of different periods in the field of image classification have discrepancies on GasHisSDB, we select a variety of classifiers for evaluation. Seven classical machine learning classifiers, three Convolutional Neural Network classifiers, and a novel transformer-based classifier are selected for testing on image classification tasks. Results: This study performed extensive experiments using traditional machine learning and deep learning methods to prove that the methods of different periods have discrepancies on GasHisSDB. Traditional machine learning achieved the best accuracy rate of 86.08% and a minimum of just 41.12%. The best accuracy of deep learning reached 96.47% and the lowest was 86.21%. Accuracy rates vary significantly across classifiers. Conclusions: To the best of our knowledge, it is the first publicly available gastric cancer histopathology dataset containing a large number of images for weakly supervised learning. We believe that GasHisSDB can attract researchers to explore new algorithms for the automated diagnosis of gastric cancer, which can help physicians and patients in the clinical setting.

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EMDS-7: Environmental Microorganism Image Dataset Seventh Version for Multiple Object Detection Evaluation

The Environmental Microorganism Image Dataset Seventh Version (EMDS-7) is a microscopic image data set, including the original Environmental Microorganism images (EMs) and the corresponding object labeling files in ".XML" format file. The EMDS-7 data set consists of 41 types of EMs, which has a total of 2365 images and 13216 labeled objects. The EMDS-7 database mainly focuses on the object detection. In order to prove the effectiveness of EMDS-7, we select the most commonly used deep learning methods (Faster-RCNN, YOLOv3, YOLOv4, SSD and RetinaNet) and evaluation indices for testing and evaluation. EMDS-7 is freely published for non-commercial purpose at: https://figshare.com/articles/dataset/EMDS-7_DataSet/16869571

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A Comprehensive Review of Image Analysis Methods for Microorganism Counting: From Classical Image Processing to Deep Learning Approaches

Microorganisms such as bacteria and fungi play essential roles in many application fields, like biotechnique, medical technique and industrial domain. Microorganism counting techniques are crucial in microorganism analysis, helping biologists and related researchers quantitatively analyze the microorganisms and calculate their characteristics, such as biomass concentration and biological activity. However, traditional microorganism manual counting methods, such as plate counting method, hemocytometry and turbidimetry, are time-consuming, subjective and need complex operations, which are difficult to be applied in large-scale applications. In order to improve this situation, image analysis is applied for microorganism counting since the 1980s, which consists of digital image processing, image segmentation, image classification and suchlike. Image analysis-based microorganism counting methods are efficient comparing with traditional plate counting methods. In this article, we have studied the development of microorganism counting methods using digital image analysis. Firstly, the microorganisms are grouped as bacteria and other microorganisms. Then, the related articles are summarized based on image segmentation methods. Each part of the article is reviewed by methodologies. Moreover, commonly used image processing methods for microorganism counting are summarized and analyzed to find common technological points. More than 144 papers are outlined in this article. In conclusion, this paper provides new ideas for the future development trend of microorganism counting, and provides systematic suggestions for implementing integrated microorganism counting systems in the future. Researchers in other fields can refer to the techniques analyzed in this paper.

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A Comparison for Patch-level Classification of Deep Learning Methods on Transparent Environmental Microorganism Images: from Convolutional Neural Networks to Visual Transformers

Nowadays, analysis of Transparent Environmental Microorganism Images (T-EM images) in the field of computer vision has gradually become a new and interesting spot. This paper compares different deep learning classification performance for the problem that T-EM images are challenging to analyze. We crop the T-EM images into 8 * 8 and 224 * 224 pixel patches in the same proportion and then divide the two different pixel patches into foreground and background according to ground truth. We also use four convolutional neural networks and a novel ViT network model to compare the foreground and background classification experiments. We conclude that ViT performs the worst in classifying 8 * 8 pixel patches, but it outperforms most convolutional neural networks in classifying 224 * 224 pixel patches.

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A Comparison for Anti-noise Robustness of Deep Learning Classification Methods on a Tiny Object Image Dataset: from Convolutional Neural Network to Visual Transformer and Performer

Image classification has achieved unprecedented advance with the the rapid development of deep learning. However, the classification of tiny object images is still not well investigated. In this paper, we first briefly review the development of Convolutional Neural Network and Visual Transformer in deep learning, and introduce the sources and development of conventional noises and adversarial attacks. Then we use various models of Convolutional Neural Network and Visual Transformer to conduct a series of experiments on the image dataset of tiny objects (sperms and impurities), and compare various evaluation metrics in the experimental results to obtain a model with stable performance. Finally, we discuss the problems in the classification of tiny objects and make a prospect for the classification of tiny objects in the future.

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A New Pairwise Deep Learning Feature For Environmental Microorganism Image Analysis

Environmental microorganism (EM) offers a high-efficient, harmless, and low-cost solution to environmental pollution. They are used in sanitation, monitoring, and decomposition of environmental pollutants. However, this depends on the proper identification of suitable microorganisms. In order to fasten, low the cost, increase consistency and accuracy of identification, we propose the novel pairwise deep learning features to analyze microorganisms. The pairwise deep learning features technique combines the capability of handcrafted and deep learning features. In this technique we, leverage the Shi and Tomasi interest points by extracting deep learning features from patches which are centered at interest points locations. Then, to increase the number of potential features that have intermediate spatial characteristics between nearby interest points, we use Delaunay triangulation theorem and straight-line geometric theorem to pair the nearby deep learning features. The potential of pairwise features is justified on the classification of EMs using SVMs, k-NN, and Random Forest classifier. The pairwise features obtain outstanding results of 99.17%, 91.34%, 91.32%, 91.48%, and 99.56%, which are the increase of about 5.95%, 62.40%, 62.37%, 61.84%, and 3.23% in accuracy, F1-score, recall, precision, and specificity respectively, compared to non-paired deep learning features.

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A Comprehensive Review of Computer-aided Whole-slide Image Analysis: from Datasets to Feature Extraction, Segmentation, Classification, and Detection Approaches

With the development of computer-aided diagnosis (CAD) and image scanning technology, Whole-slide Image (WSI) scanners are widely used in the field of pathological diagnosis. Therefore, WSI analysis has become the key to modern digital pathology. Since 2004, WSI has been used more and more in CAD. Since machine vision methods are usually based on semi-automatic or fully automatic computers, they are highly efficient and labor-saving. The combination of WSI and CAD technologies for segmentation, classification, and detection helps histopathologists obtain more stable and quantitative analysis results, save labor costs and improve diagnosis objectivity. This paper reviews the methods of WSI analysis based on machine learning. Firstly, the development status of WSI and CAD methods are introduced. Secondly, we discuss publicly available WSI datasets and evaluation metrics for segmentation, classification, and detection tasks. Then, the latest development of machine learning in WSI segmentation, classification, and detection are reviewed continuously. Finally, the existing methods are studied, the applicabilities of the analysis methods are analyzed, and the application prospects of the analysis methods in this field are forecasted.

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