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Marco Pascucci

Publications and source records attributed to Marco Pascucci.

2 recordsLinked to original sources

Change-in-Slope Optimal Partitioning Algorithm in a Finite-Size Parameter Space

We consider the problem of detecting change-points in univariate time series by fitting a continuous piecewise linear signal using the residual sum of squares. Values of the inferred signal at slope breaks are restricted to a finite set of size $m$. Using this finite parameter space, we build a dynamic programming algorithm with a controlled time complexity of $O(m^2n^2)$ for $n$ data points. Some accelerating strategies can be used to reduce the constant before $n^2$. The adapted classic inequality-based pruning is outperformed by a simpler "channel" method on simulations. Besides, our finite parameter space setting allows an easy introduction of constraints on the inferred signal. For example, imposing a minimal angle between consecutive segment slopes provides robustness to model misspecification and outliers. We test our algorithm with an isotonic constraint on an antibiogram image analysis problem for which algorithmic efficiency is a cornerstone in the emerging context of mobile-health and embedded medical devices. For this application, a finite state approach can be a valid compromise.

stat.CO

Compressive three-dimensional super-resolution microscopy with speckle-saturated fluorescence excitation

Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination patterns are combined with the saturation of an optical transition to enable sub-diffraction imaging. While the technique proved useful for two-dimensional imaging, extending it to three-dimensions (3D) is challenging due to the fading/fatigue of organic fluorophores under intense cycling conditions. Here, we present a compressed sensing approach that allows for the first time 3D sub-diffraction nSIM of cultured cells by saturating fluorescence excitation. Exploiting the natural orthogonality of transverse speckle illumination planes, 3D probing of the sample is achieved by a single two-dimensional scan. Fluorescence contrast under saturated excitation is ensured by the inherent high density of intensity minima associated with optical vortices in polarized speckle patterns. Compressed speckle microscopy is thus a simple approach that enables 3D super-resolved nSIM imaging with potentially considerably reduced acquisition time and photobleaching.les fast 3D super-resolved imaging with considerably minimized photo-bleaching.

physics.optics