arXiv subjects

Maria Kapetanaki

Publications and source records attributed to Maria Kapetanaki.

2 recordsLinked to original sources

arXiv2026

DiSTILL: A Hybrid Cloud-HPC Workflow System for Reproducible Spatial Transcriptomics Analysis

Spatial transcriptomics workflows increasingly combine large annotated data objects, notebook-based analyses, and resource-intensive statistical models that must be executed on high-performance computing (HPC) systems. In practice, these workflows are often difficult to reproduce because configuration, validation, stage execution, and artifact handling are fragmented across $\textit{ad hoc}$ scripts and manually edited notebooks. We present $\textit{DiSTILL}$ (Disease Diagnosis from Spatial Transcriptomics via Interpretable Latent Learning), a hybrid cloud$-$HPC workflow system for reproducible spatial transcriptomics (ST) analysis. DiSTILL combines an application programming interface (API) backend built with $\texttt{FastAPI}$, a web frontend, a dataset and preset registry, and a Python pipeline generator that materializes run-specific execution bundles and $\texttt{SLURM}$ submission scripts. The system supports local, Secure Shell (SSH)-mediated, and pull-based poller execution modes, enabling HPC submission in environments where persistent API-initiated automation is restricted. We describe the system through the lens of an inflammatory bowel disease (IBD) ST workflow that operationalizes the analytical pipeline of Tan $\textit{et al.}$ into an auditable application layer. Accordingly, the contribution of this paper is a workflow systems contribution centered on reproducible execution, queue-based orchestration, configuration semantics, and deployment across a split cloud$-$HPC architecture. The broader application goal of DiSTILL is to support user-supplied datasets that satisfy the schema assumptions of the wrapped analytical pipeline.

q-bio.GN↗

arXiv2026

Engineering Spatial and Molecular Features from Cellular Niches to Inform Predictions of Inflammatory Bowel Disease

Differentiating between the two main subtypes of Inflammatory Bowel Disease (IBD): Crohns disease (CD) and ulcerative colitis (UC) is a persistent clinical challenge due to overlapping presentations. This study introduces a novel computational framework that employs spatial transcriptomics (ST) to create an explainable machine learning model for IBD classification. We analyzed ST data from the colonic mucosa of healthy controls (HC), UC, and CD patients. Using Non-negative Matrix Factorization (NMF), we first identified four recurring cellular niches, representing distinct functional microenvironments within the tissue. From these niches, we systematically engineered 44 features capturing three key aspects of tissue pathology: niche composition, neighborhood enrichment, and niche-gene signals. A multilayer perceptron (MLP) classifier trained on these features achieved an accuracy of $0.774 \pm 0.161$ for the more challenging three-class problem (HC, UC, and CD) and $0.916 \pm 0.118$ in the two-class problem of distinguishing IBD from healthy tissue. Crucially, model explainability analysis revealed that disruptions in the spatial organization of niches were the strongest predictors of general inflammation, while the classification between UC and CD relied on specific niche-gene expression signatures. This work provides a robust, proof-of-concept pipeline that transforms descriptive spatial data into an accurate and explainable predictive tool, offering not only a potential new diagnostic paradigm but also deeper insights into the distinct biological mechanisms that drive IBD subtypes.

q-bio.GN↗