Maryam Saadatmand | Searcharxiv

arXiv2024

Microfluidic 3D Cell Culture: Potential Application of Collagen Hydrogels with an Optimal Dose of Bioactive Glasses

We engineered a microfluidic platform to study the effects of bioactive glass nanoparticles (BGNs) on cell viability under static culture. We incorporated different concentrations of BGNs (1%, 2%, and 3% w/v) in collagen hydrogel (with a concentration of 3.0 mg/mL). The microfluidic chip's dimensions were optimized through fluid flow and mass transfer simulations. Collagen type I extracted from rat tail tendons was used as the main material, and BGNs synthesized by the sol-gel method were used to enhance the mechanical properties of the hydrogel. The extracted collagen was characterized using FTIR and SDS-PAGE, and BGNs were analyzed using XRD, FTIR, DLS, and FE-SEM/EDX. The structure of the collagen-BGNs hydrogels was examined using SEM, and their mechanical properties were determined using rheological analysis. The cytotoxicity of BGNs was assessed using the MTT assay, and the viability of fibroblast (L929) cells encapsulated in the collagen-BGNs hydrogel inside the microfluidic device was assessed using a live/dead assay. Based on all these test results, the L929 cells showed high cell viability in vitro and promising microenvironment mimicry in a microfluidic device. Collagen3-BGNs3 (Collagen 3 mg/mL + BGNs 3% (w/v)) was chosen as the most suitable sample for further research on a microfluidic platform.

q-bio.TO↗

arXiv2020

Computational study of geometric effects of bottom wall microgrooves on cell docking inside microfluidic devices

Single cell and regularly cells docking inside the microstructure of microfluidic systems are advantageous in different analyses of single cells exposed to equal drug concentration and mechanical stimulus. In this study, we investigated bottom wall microgrooves with semicircular and rectangular geometries with different sizes which are suitable for single cell docking along the length of the microgroove in x direction and numerous cells docking regularly in one line inside the microgroove in a 3D microchannel. We used computational fluid dynamics to analyze the fluid recirculation area inside different microgrooves. The height of recirculation area in the bottom of microgroove can affect the cell attachment, and also materials delivery to attached cells, so the height of recirculation area has to be optimum amount. In addition, we analyzed the fluid drag force on cell movement toward the microgroove. This parameter was proportional to the fluid velocities in x and y directions changing in different microgrooves geometries. In different microgrooves geometries the fluid velocity in y direction does not change. If the fluid velocity in x direction decreases inside the microgroove, the cell movement time inside the microgroove will increase, and also the drag force in y direction can push the cells toward the bottom due to the lower drag force in x direction. The percentages of negative shear stress and average shear stress on the adhered cell surface were also calculated. The lower average shear stress, and negative shear stress around 50% on the cell surface are against cell detachment from the substrate. The results indicated that at the constant fluid inlet velocity and microchannel height, microgroove geometry and ratio of cell size to the microgroove size play pivotal roles in the cell initial adhesion to the substrate as well as the cell detachment.

physics.bio-ph↗