arXiv subjects

Michael F. Schmid

Publications and source records attributed to Michael F. Schmid.

2 recordsLinked to original sources

arXiv2023

Improving Resolution and Resolvability of Single Particle CryoEM using Gaussian Mixture Models

Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here, we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation, and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures.

q-bio.QM↗

arXiv2017

Convolutional Neural Networks for Automated Annotation of Cellular Cryo-Electron Tomograms

Cellular Electron Cryotomography (CryoET) offers the ability to look inside cells and observe macromolecules frozen in action. A primary challenge for this technique is identifying and extracting the molecular components within the crowded cellular environment. We introduce a method using neural networks to dramatically reduce the time and human effort required for subcellular annotation and feature extraction. Subsequent subtomogram classification and averaging yields in-situ structures of molecular components of interest.

q-bio.QM↗