Functional analysis of a gene locus in response to non-canonical combinations of transcription factors
Transcription factor combinations determine gene locus activity and thereby cell identity. However, the precise link between concentrations of such activating transcription factors and target-gene activity is ambiguous. Here we investigate this link for the gap gene dependent activation of the even-skipped (eve) locus in the Drosophila embryo. We simultaneously measure the spatiotemporal gap gene concentrations in hemizygous and homozygous gap mutants, and link these to eve activity. Although changes in expression extend well beyond the genetically manipulated gene, nearly all expression alternations approximate the canonical combinations of activating levels in wild-type, sometimes necessitating pattern shifts. Expression levels that diverge from the wild-type repertoire still drive locus activation. Specific stripes in the homozygous mutants show partial penetrance, justifying their renown variable phenotypes. However, all eve stripes appear at highly reproducible positions, even though a broader span of gap gene expression levels activates eve. Our results suggest a correction capacity of the gap gene network and set constraints on the activity of multi-enhancer gene loci.