Super-resolution imaging within reach
Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain difficult to build or are photo-toxic for the biological samples. However, microsphere-assisted microscopy has recently made super-resolution imaging accessible to scientists (e.g. optical metrologists, engineers and biologists). This paper presents an easy-to-implement optical setup to perform full-field and contactless super-resolution measurements of nanostructured media or biological elements. For this purpose, a classical microscope was enhanced by introducing a transparent microsphere. We show that this rather simple approach makes it possible to achieve a lateral resolution of 200 nm in air, i.e. the visualization of feature sizes of 100 nm.