arXiv subjects

Qianwan Yang

Publications and source records attributed to Qianwan Yang.

4 recordsLinked to original sources

arXiv2026

Coordinate-conditioned Deconvolution for Scalable Spatially Varying High-Throughput Imaging

Wide-field fluorescence microscopy with compact optics often suffers from spatially varying blur due to field-dependent aberrations, vignetting, and sensor truncation, while finite sensor sampling imposes an inherent trade-off between field of view (FOV) and resolution. Computational Miniaturized Mesoscope (CM2) alleviate the sampling limit by multiplexing multiple sub-views onto a single sensor, but introduce view crosstalk and a highly ill-conditioned inverse problem compounded by spatially variant point spread functions (PSFs). Prior learning-based spatially varying (SV) reconstruction methods typically rely on global SV operators with fixed input sizes, resulting in memory and training costs that scale poorly with image dimensions. We propose SV-CoDe (Spatially Varying Coordinate-conditioned Deconvolution), a scalable deep learning framework that achieves uniform, high-resolution reconstruction across a 6.5 mm FOV. Unlike conventional methods, SV-CoDe employs coordinate-conditioned convolutions to locally adapt reconstruction kernels; this enables patch-based training that decouples parameter count from FOV size. SV-CoDe achieves the best image quality in both simulated and experimental measurements while requiring 10x less model size and 10x less training data than prior baselines. Trained purely on physics-based simulations, the network robustly generalizes to bead phantoms, weakly scattering brain slices, and freely moving C. elegans. SV-CoDe offers a scalable, physics-aware solution for correcting SV blur in compact optical systems and is readily extendable to a broad range of biomedical imaging applications.

eess.IV↗

arXiv2024

Wide-Field, High-Resolution Reconstruction in Computational Multi-Aperture Miniscope Using a Fourier Neural Network

Traditional fluorescence microscopy is constrained by inherent trade-offs among resolution, field-of-view, and system complexity. To navigate these challenges, we introduce a simple and low-cost computational multi-aperture miniature microscope, utilizing a microlens array for single-shot wide-field, high-resolution imaging. Addressing the challenges posed by extensive view multiplexing and non-local, shift-variant aberrations in this device, we present SV-FourierNet, a novel multi-channel Fourier neural network. SV-FourierNet facilitates high-resolution image reconstruction across the entire imaging field through its learned global receptive field. We establish a close relationship between the physical spatially-varying point-spread functions and the network's learned effective receptive field. This ensures that SV-FourierNet has effectively encapsulated the spatially-varying aberrations in our system, and learned a physically meaningful function for image reconstruction. Training of SV-FourierNet is conducted entirely on a physics-based simulator. We showcase wide-field, high-resolution video reconstructions on colonies of freely moving C. elegans and imaging of a mouse brain section. Our computational multi-aperture miniature microscope, augmented with SV-FourierNet, represents a major advancement in computational microscopy and may find broad applications in biomedical research and other fields requiring compact microscopy solutions.

physics.optics↗

arXiv2024

EventLFM: Event Camera integrated Fourier Light Field Microscopy for Ultrafast 3D imaging

Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed and space-bandwidth product (SBP). Emerging single-shot 3D wide-field techniques offer a promising alternative but are bottlenecked by the synchronous readout constraints of conventional CMOS systems, thus restricting data throughput to maintain high SBP at limited frame rates. To address this, we introduce EventLFM, a straightforward and cost-effective system that overcomes these challenges by integrating an event camera with Fourier light field microscopy (LFM), a state-of-the-art single-shot 3D wide-field imaging technique. The event camera operates on a novel asynchronous readout architecture, thereby bypassing the frame rate limitations inherent to conventional CMOS systems. We further develop a simple and robust event-driven LFM reconstruction algorithm that can reliably reconstruct 3D dynamics from the unique spatiotemporal measurements captured by EventLFM. Experimental results demonstrate that EventLFM can robustly reconstruct fast-moving and rapidly blinking 3D fluorescent samples at kHz frame rates. Furthermore, we highlight EventLFM's capability for imaging of blinking neuronal signals in scattering mouse brain tissues and 3D tracking of GFP-labeled neurons in freely moving C. elegans. We believe that the combined ultrafast speed and large 3D SBP offered by EventLFM may open up new possibilities across many biomedical applications.

physics.optics↗

arXiv2022

Deep-learning-augmented Computational Miniature Mesoscope

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of miniaturized platforms providing micron-scale resolution across centimeter-scale FOVs. To overcome this challenge, we developed Computational Miniature Mesoscope (CM$^2$) that exploits a computational imaging strategy to enable single-shot 3D high-resolution imaging across a wide FOV in a miniaturized platform. Here, we present CM$^2$ V2 that significantly advances both the hardware and computation. We complement the 3$\times$3 microlens array with a new hybrid emission filter that improves the imaging contrast by 5$\times$, and design a 3D-printed freeform collimator for the LED illuminator that improves the excitation efficiency by 3$\times$. To enable high-resolution reconstruction across the large imaging volume, we develop an accurate and efficient 3D linear shift-variant (LSV) model that characterizes the spatially varying aberrations. We then train a multi-module deep learning model, CM$^2$Net, using only the 3D-LSV simulator. We show that CM$^2$Net generalizes well to experiments and achieves accurate 3D reconstruction across a $\sim$7-mm FOV and 800-$μ$m depth, and provides $\sim$6-$μ$m lateral and $\sim$25-$μ$m axial resolution. This provides $\sim$8$\times$ better axial localization and $\sim$1400$\times$ faster speed as compared to the previous model-based algorithm. We anticipate this simple and low-cost computational miniature imaging system will be impactful to many large-scale 3D fluorescence imaging applications.

physics.optics↗