arXiv subjects

Robert M. Glaeser

Publications and source records attributed to Robert M. Glaeser.

5 recordsLinked to original sources

arXiv2024

Modern approaches to improving phase contrast electron microscopy

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a phase shift to the unscattered part of the electron beam. Many approaches have been investigated, including the ponderomotive interaction between light and electrons. We review the recent successes achieved with this method in high-resolution, single-particle cryo-EM. We also review the status of using pulsed or near-field enhanced laser light as alternatives, along with approaches that use scanning transmission electron microscopy (STEM) with a segmented detector rather than a phase plate.

q-bio.QM↗

arXiv2020

High-Power Near-Concentric Fabry-Perot Cavity for Phase Contrast Electron Microscopy

Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous-wave intensities of over 450 GW/cm$^{2}$, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.

physics.ins-det↗

arXiv2020

Observation of the relativistic reversal of the ponderomotive potential

The secular dynamics of a non-relativistic charged particle in an electromagnetic wave can be described by the ponderomotive potential. Although ponderomotive electron-laser interactions at relativistic velocities are important for emerging technologies from laser-based particle accelerators to laser-enhanced electron microscopy, the effects of special relativity on the interaction have only been studied theoretically. Here, we use a transmission electron microscope to measure the position-dependent phase shift imparted to a relativistic electron wave function when it traverses a standing laser wave. The kinetic energy of the electrons is varied between $80\,\mathrm{keV}$ and $300\,\mathrm{keV}$, and the laser standing wave has a continuous-wave intensity of $175\,\mathrm{GW/cm}^2$. In contrast to the non-relativistic case, we demonstrate that the phase shift depends on both the electron velocity and the wave polarization, confirming the predictions of a quasiclassical theory of the interaction. Remarkably, if the electron's speed is greater than $1/\sqrt{2}$ of the speed of light, the phase shift at the electric field nodes of the wave can exceed that at the antinodes. In this case there exists a polarization such that the phase shifts at the nodes and antinodes are equal, and the electron does not experience Kapitza-Dirac diffraction. Our results thus provide new capabilities for coherent electron beam manipulation.

physics.optics↗

arXiv2016

Continuous 40 GW/cm$^2$ laser intensity in a near-concentric optical cavity

Manipulating free-space electron wave functions with laser fields can bring about new electron-optical elements for transmission electron microscopy. In particular, a Zernike phase plate would enable high-contrast imaging of soft matter, leading to new opportunities in structural biology and materials science. A Zernike plate can be implemented using a tight, intense CW laser focus that shifts the phase of the electron wave by the ponderomotive potential. Here, we use a near-concentric cavity to focus 7.5 kW of circulating laser power at 1064 nm into a 7 $μ$m waist, setting a record for CW laser intensity and establishing a pathway to ponderomotive phase contrast TEM.

physics.optics↗

arXiv2010

Design of an electron microscope phase plate using a focused continuous-wave laser

We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the relativistic quantum theory of the phase shift caused by the laser-electron-interaction, study resonant cavities for enhancing the laser intensity, and discuss applications in biology, soft materials science, and atomic and molecular physics.

physics.ins-det↗