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Rui Reis

Publications and source records attributed to Rui Reis.

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Using Medical Algorithms for Task-Oriented Dialogue in LLM-Based Medical Interviews

We developed a task-oriented dialogue framework structured as a Directed Acyclic Graph (DAG) of medical questions. The system integrates: (1) a systematic pipeline for transforming medical algorithms and guidelines into a clinical question corpus; (2) a cold-start mechanism based on hierarchical clustering to generate efficient initial questioning without prior patient information; (3) an expand-and-prune mechanism enabling adaptive branching and backtracking based on patient responses; (4) a termination logic to ensure interviews end once sufficient information is gathered; and (5) automated synthesis of doctor-friendly structured reports aligned with clinical workflows. Human-computer interaction principles guided the design of both the patient and physician applications. Preliminary evaluation involved five physicians using standardized instruments: NASA-TLX (cognitive workload), the System Usability Scale (SUS), and the Questionnaire for User Interface Satisfaction (QUIS). The patient application achieved low workload scores (NASA-TLX = 15.6), high usability (SUS = 86), and strong satisfaction (QUIS = 8.1/9), with particularly high ratings for ease of learning and interface design. The physician application yielded moderate workload (NASA-TLX = 26) and excellent usability (SUS = 88.5), with satisfaction scores of 8.3/9. Both applications demonstrated effective integration into clinical workflows, reducing cognitive demand and supporting efficient report generation. Limitations included occasional system latency and a small, non-diverse evaluation sample.

cs.AI

The long non-coding RNA HOTAIR is transcriptionally activated by HOXA9 and is an independent prognostic marker in patients with malignant glioma

The lncRNA HOTAIR has been implicated in several human cancers. Here, we evaluated the molecular alterations and upstream regulatory mechanisms of HOTAIR in glioma, the most common primary brain tumors, and its clinical relevance. HOTAIR gene expression, methylation, copy-number and prognostic value were investigated in human gliomas integrating data from online datasets and our cohorts. High levels of HOTAIR were associated with higher grades of glioma, particularly IDH wild-type cases. Mechanistically, HOTAIR was overexpressed in a gene dosage-independent manner, while DNA methylation levels of particular CpGs in HOTAIR locus were associated with HOTAIR expression levels in GBM clinical specimens and cell lines. Concordantly, the demethylating agent 5-Aza-2'-deoxycytidine affected HOTAIR transcriptional levels in a cell line-dependent manner. Importantly, HOTAIR was frequently co-expressed with HOXA9 in high-grade gliomas from TCGA, Oncomine, and our Portuguese and French datasets. Integrated in silico analyses, chromatin immunoprecipitation, and qPCR data showed that HOXA9 binds directly to the promoter of HOTAIR. Clinically, GBM patients with high HOTAIR expression had a significantly reduced overall survival, independently of other prognostic variables. In summary, this work reveals HOXA9 as a novel direct regulator of HOTAIR, and establishes HOTAIR as an independent prognostic marker, providing new therapeutic opportunities to treat this highly aggressive cancer.

q-bio.GN